OXA-48 - Science

Early detection of OXA-48 producing Klebsiella pneumoniae with the use of rapid antibiotic susceptibility testing

De Muynck E., Vandendriessche S., Messiaen A-S., Decommer K., Huis In 't Veld D., J De Waele J., Boelens J.
Eur J Clin Microbiol Infect Dis, 2024 Dec 19.

Abstract
Rapid antimicrobial susceptibility testing of positive blood cultures can enhance antimicrobial stewardship and patient outcomes. We present a case where OXA-48-producing Klebsiella pneumoniae with low-level carbapenem resistance was suspected 6 h after blood-culture positivity, based on ASTar system (Q-Linea, Sweden) results. OXA-48 carbapenemase presence was confirmed by the OXA-48 K-SeT lateral flow assay (Coris, Belgium) on a short-term subculture. This led to timely therapeutic adjustments. Following this case, we validated the OXA-48 K-SeT and Xpert Carba-R assay (Cepheid, USA) on short-term incubated (6 h) subcultures of positive blood cultures, demonstrating that OXA-48 K-SeT and Xpert Carba-R respectively accurately identified OXA-48 and various (OXA-48, VIM, NDM, KPC, IMP) carbapenemase-producing Enterobacterales (CPE) types. Both assays prove valuable for rapid CPE detection in clinical settings.

https://link.springer.com/article/10.1007/s10096-024-05007-2

The Global Ascendency of OXA-48-Type Carbapenemases

Pitout JDD, Peirano G, Kock MM, Strydom KA, Matsumura Y
Clin Microbiol Rev. 2019 Nov 13;33(1):e00102-19

Abstract
Surveillance studies have shown that OXA-48-like carbapenemases are the most common carbapenemases in Enterobacterales in certain regions of the world and are being introduced on a regular basis into regions of nonendemicity, where they are responsible for nosocomial outbreaks. OXA-48, OXA-181, OXA-232, OXA-204, OXA-162, and OXA-244, in that order, are the most common enzymes identified among the OXA-48-like carbapenemase group. OXA-48 is associated with different Tn1999 variants on IncL plasmids and is endemic in North Africa and the Middle East. OXA-162 and OXA-244 are derivatives of OXA-48 and are present in Europe. OXA-181 and OXA-232 are associated with ISEcp1, Tn2013 on ColE2, and IncX3 types of plasmids and are endemic in the Indian subcontinent (e.g., India, Bangladesh, Pakistan, and Sri Lanka) and certain sub-Saharan African countries. Overall, clonal dissemination plays a minor role in the spread of OXA-48-like carbapenemases, but certain high-risk clones (e.g., Klebsiella pneumoniae sequence type 147 [ST147], ST307, ST15, and ST14 and Escherichia coli ST38 and ST410) have been associated with the global dispersion of OXA-48, OXA-181, OXA-232, and OXA-204. Chromosomal integration of bla OXA-48 within Tn6237 occurred among E. coli ST38 isolates, especially in the United Kingdom. The detection of Enterobacterales with OXA-48-like enzymes using phenotypic methods has improved recently but remains challenging for clinical laboratories in regions of nonendemicity. Identification of the specific type of OXA-48-like enzyme requires sequencing of the corresponding genes. Bacteria (especially K. pneumoniae and E. coli) with bla OXA-48, bla OXA-181, and bla OXA-232 are emerging in different parts of the world and are most likely underreported due to problems with the laboratory detection of these enzymes. The medical community should be aware of the looming threat that is posed by bacteria with OXA-48-like carbapenemases.

https://pubmed.ncbi.nlm.nih.gov/31722889/

Genetic and Biochemical Characterization of OXA-535, a Distantly Related OXA-48-Like β-Lactamase.

Dabos L, Jousset AB, Bonnin RA, Fortineau N, Zavala A, Retailleau P, Iorga BI, Naas T.
Antimicrob Agents Chemother. 2018 Sep 24;62(10). pii: e01198-18.

Abstract
OXA-535 is a chromosome-encoded carbapenemase of Shewanella bicestrii JAB-1 that shares only 91.3% amino acid sequence identity with OXA-48. Catalytic efficiencies are similar to those of OXA-48 for most β-lactams, except for ertapenem, where a 2,000-fold-higher efficiency was observed with OXA-535. OXA-535 and OXA-436, a plasmid-encoded variant of OXA-535 differing by three amino acids, form a novel cluster of distantly related OXA-48-like carbapenemases. Comparison of blaOXA-535 and blaOXA-436 genetic environments suggests that an ISCR1 may be responsible for blaOXA-436 gene mobilization from the chromosome of Shewanella spp. to plasmids.

https://www.ncbi.nlm.nih.gov/pubmed/30082287

P1064 – Rectal screening of OXA-48-producing Enterobacteriaceae.

S.A. Gibaud, E.Thomas, L. Crémet and J. Caillon.
28th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 21 – 24, 2018
Poster

Chromogenic culture media or rapid immunochromatographic test: Which is better for detecting Klebsiella pneumoniae that produce OXA-48 and can they be used in blood and urine specimens.

Genc O., Aksub E.
J Microbiol Methods 2018 148: 169–173.

Abstract
Our goal was to compare a rapid test (OXA-48K-SeT) and four different chromogenic media (CHROMagar KPC, CHROMagar mSuperCARBA, ChromID Carba and ChromID OXA-48) for the detection of OXA-48 producing Klebsiella pneumoniae isolates and spiked urine/blood samples with these bacteria. In total 100 K.pneumoniae isolates, including 60 OXA-48 positive, 15 other carbapenemase producing, 15 Extended spectrum betalactamases (ESBL) positive and 10 carbapenem sensitive K.pneumoniae were included in the study. After all samples were inoculated into all chromogenic media, temocillin discs were placed onto the media. OXA-48K-SeT was studied according to the manufacturer's instructions and the lower detection limit was determined. Sensitivities and specificities of all chromogenic media and rapid test were detected as 100%. All of the OXA-48 producers were found resistant to temocillin on all chromogenic media. The lower detection limit of the rapid assay was determined as 106 in both direct bacterial samples and in spiked urine/blood samples. As a result, four chromogenic culture media and OXA-48 K-SeT can be used safely for detection of OXA-48 positive K.pneumoniae isolates. Although direct clinical specimens were not used, our study suggests that this media and OXA-48 K-SeT may be used in patient samples like blood and urine. Further studies are needed to assess this suggestion.

https://www.ncbi.nlm.nih.gov/pubmed/29679603

CTX-M-15-Producing Shewanella Species Clinical Isolate Expressing OXA-535, a Chromosome-Encoded OXA-48 Variant, Putative Progenitor of the Plasmid-Encoded OXA-436.

Jousset AB, Dabos L, Bonnin RA, Girlich D, Potron A, Cabanel N, Dortet L, Glaser P, Naas T.
Antimicrob Agents Chemother. 2017 Dec 21;62(1). pii: e01879-17.

Abstract
Shewanella spp. constitute a reservoir of antibiotic resistance determinants. In a bile sample, we identified three extended-spectrum-β-lactamase (ESBL)-producing bacteria (Escherichia coli, Klebsiella pneumoniae, and Shewanella sp. strain JAB-1) isolated from a child suffering from cholangitis. Our objectives were to characterize the genome and the resistome of the first ESBL-producing isolate of the genus Shewanella and determine whether plasmidic exchange occurred between the three bacterial species. Bacterial isolates were characterized using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), standard biochemical tools, and antimicrobial susceptibility testing. Shewanella sp. JAB-1 and ESBL gene-encoding plasmids were characterized using PacBio and Illumina whole-genome sequencing, respectively. The Shewanella sp. JAB-1 chromosome-encoded OXA-48 variant was cloned and functionally characterized. Whole-genome sequencing (WGS) of the Shewanella sp. clinical isolate JAB-1 revealed the presence of a 193-kb plasmid belonging to the IncA/C incompatibility group and harboring two ESBL genes, blaCTX-M-15 and blaSHV-2ablaCTX-M-15 gene-carrying plasmids belonging to the IncY and IncR incompatibility groups were also found in the E. coli and K. pneumoniae isolates from the same patient, respectively. A comparison of the blaCTX-M-15 genetic environment indicated the independent origin of these plasmids and dismissed in vivo transfers. Furthermore, characterization of the resistome of Shewanella sp. JAB-1 revealed the presence of a chromosome-carried blaOXA-535 gene, likely the progenitor of the plasmid-carried blaOXA-436 gene, a novel blaOXA-48-like gene. The expression of blaOXA-535 in E. coli showed the carbapenem-hydrolyzing activity of OXA-535. The production of OXA-535 in Shewanella sp. JAB-1 could be evidenced using molecular and immunoenzymatic tests, but not with biochemical tests that monitor carbapenem hydrolysis. In this study, we have identified a CTX-M-15-producing Shewanella species that was responsible for a hepatobiliary infection and that is likely the progenitor of OXA-436, a novel plasmid-encoded OXA-48-like class D carbapenemase.

https://www.ncbi.nlm.nih.gov/pubmed/29038283

Dissemination and Characteristics of a Novel Plasmid-Encoded Carbapenem-Hydrolyzing Class D β-Lactamase, OXA-436, Found in Isolates from Four Patients at Six Different Hospitals in Denmark.

Samuelsen Ø, Hansen F, Aasnæs B, Hasman H, Lund BA, Leiros HS, Lilje B, Janice J, Jakobsen L, Littauer P, Søes LM, Holzknecht BJ, Andersen LP, Stegger M, Andersen PS and  Hammerum AM
Antimicrob Agents Chemother. 2017 Dec 21;62(1) :1260-17.

Abstract
The diversity of OXA-48-like carbapenemases is continually expanding. In this study, we describe the dissemination and characteristics of a novel carbapenem-hydrolyzing class D β-lactamase (CHDL) named OXA-436. In total, six OXA-436-producing Enterobacteriaceae isolates, including Enterobacter asburiae (n = 3), Citrobacter freundii (n = 2), and Klebsiella pneumoniae (n = 1), were identified in four patients in the period between September 2013 and April 2015. All three species of OXA-436-producing Enterobacteriaceae were found in one patient. The amino acid sequence of OXA-436 showed 90.4 to 92.8% identity to the amino acid sequences of other acquired OXA-48-like variants. Expression of OXA-436 in Escherichia coli and kinetic analysis of purified OXA-436 revealed an activity profile similar to that of OXA-48 and OXA-181, with activity against penicillins, including temocillin; limited or no activity against extended-spectrum cephalosporins; and activity against carbapenems. The blaOXA-436 gene was located on a conjugative ∼314-kb IncHI2/IncHI2A plasmid belonging to plasmid multilocus sequence typing sequence type 1 in a region surrounded by chromosomal genes previously identified to be adjacent to blaOXA genes in Shewanella spp. In conclusion, OXA-436 is a novel CHDL with functional properties similar to those of OXA-48-like CHDLs. The described geographical spread among different Enterobacteriaceae and the plasmid location of blaOXA-436 illustrate its potential for further dissemination.

https://www.ncbi.nlm.nih.gov/pubmed/29061750

OXA-244-Producing Escherichia coli isolates, a Challenge for clinical microbiology laboratories.

Hoyos-Mallecot Y, Naas T,  Bonnin R, Patino R, Glaser P,  Fortineau N and Dortet L.
Antimicrob Agents Chemother. 2017 Aug 24;61(9): e00818-17.

Abstract
OXA-244 is a single-point-mutant derivative of OXA-48 displaying reduced carbapenemase activity. Here, we report the microbiological features of seven OXA-244-producing Escherichia coli isolates. Only one isolate grew on ChromID Carba Smart medium (bioMérieux), but six of the seven isolates grew on ChromID extended-spectrum-β-lactamase (ESBL) medium (bioMérieux), as they coproduced an ESBL and/or a plasmid-encoded cephalosporinase. The production of a carbapenemase was detected in 57.1%, 71.4%, 71.4%, and 100% of the E. coli isolates using the Carba NP test, the Rapidec Carba NP test (bioMérieux), a matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) hydrolysis assay (Bruker), and the OXA-48 K-SeT assay (Coris BioConcept), respectively. Our results indicate that OXA-244-producing E. coli isolates are difficult to detect, which may lead to their silent spread.

https://www.ncbi.nlm.nih.gov/pubmed/28674064

Detection of OXA-370 directly from rectal swabs and blood culture vials using an immunochromatographic assay.

Nodari CS, Gales AC, Barth AL, Magagnin CM, Zavascki AP, Carvalhaes CG.
J Microbiol Methods. 2017 May 5;139:92-94. doi: 10.1016/j.mimet.2017.05.003.

Abstract
We evaluated the performance of OXA-48 K-SeT assay for detecting OXA-370 directly from spiked rectal swabs and blood culture vials. The limit of detection of this test was 104UFC/mL for rectal swabs. Detection of the OXA-370-producing isolates was successfully achieved directly from positive blood culture vials independent of growing conditions.

http://www.sciencedirect.com/science/article/pii/S0167701217301136

P0292 – Evaluation of five commercial confirmation tests for Carbapenemase-Producing
Enterobacteriaceaein an OXA-48 endemic geographic region.

C. Trouvé, R. De Smedt and E. De Laere
27th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 22 – 25, 2017
Poster

EV0199 – Rapid and accurate detection of OXA-48 like carbapenemases in Enterobacteriaceae using the OXA-48 K-SeT kit.

F. Ismail, P. Meidany, M. Kock, M. Said, N. Mbelle and KA Strydom
27th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 22 – 25, 2017
Poster

Comparison of the Novel Oxa-48 and Kpc K-SeT Assay, and Blue-Carba Test for the Detection of Carbapenemase-Producing Enterobacteriaceae Using PCR as a Reference Method.

Erdem F, Abulaila A, Aktas Z, Oncul O.
Clin Lab. 2017 Mar 1;63(3):515-522. doi: 10.7754/Clin.Lab.2016.160911.

Abstract

BACKGROUND:

Rapid, simple, and accurate laboratory detection of carbapenemases is very important for proper antibiotic therapy and infection control.

METHODS:

In this study, carbapenem-nonsusceptible Enterobacteriaceae (CRE) isolates were used to evaluate the performance of a new lateral flow immunochromatographic (IC) assay, the OXA-48 and KPC K-SeT assay, and modified Blue-Carba test (BCT) for the rapid detection of OXA-48 carbapenemase in comparison with polymerase chain reaction (PCR) amplification. These CREs of various enterobacterial species were isolated from various clinical samples including OXA-48 (47), NDM-1 (6), KPC-1 (1), IMP-1 (1), VIM-2,-4 (2), IMP-2 (1), OXA-51 (1), and OXA-23 (1) producers.

RESULTS:

The OXA-48 K-SeT test detected all OXA-48 carbapenemase producers with 100% sensitivity and specificity. The BCT detected carbapenemase producers with 93% sensitivity and 100% specificity.

CONCLUSIONS:

Both IC assays and BCT tests have good performance and are easy to perform, rapid, simple to interpret, and highly sensitive. We suggest that BCT can be used initially as an accurate, inexpensive, and rapid phenotypic confirmation test to identify Class A, B, and D

https://www.ncbi.nlm.nih.gov/pubmed/28271693

Colistin- and carbapenem-resistant Klebsiella oxytoca harboring blaVIM-2 and an insertion in the mgrB gene isolated from blood culture.

Simon M, Melzl H, Hiergeist A, Richert K, Falgenhauer L, Pfeifer Y, Gerlach RG, Fuchs K, Reischl U, Gessner A, Jantsch J.
Int J Med Microbiol. 2017 Feb;307(2):113-115

Abstract

A carbapenemase-producing colistin-resistant Klebsiella oxytoca isolate was recovered from a blood culture of a female patient without previous report of risk factors to obtain multidrug-resistant Gram-negative bacilli. A combination of biochemical and molecular methods was used to identify the resistance mechanism of this isolate. Carbapenemase production was mediated by Verona integron-encoded metallo-β-lactamase (VIM)-2. Colistin resistance was not due to plasmid- borne mcr-1 gene, but we found an integration of IS5-like sequence in the mgrB gene of K. oxytoca. This gene is known to be an important regulator of the PhoPQ two-component system, and the disruption of this gene is most likely the cause of lipid A modification resulting in colistin resistance of our isolate. To the best of our knowledge this constitutes the first report of a carbapenemase-producing K. oxytoca with colistin resistance, a case that demonstrates the limited treatment options for infections with multidrug-resistant organisms.

https://www.ncbi.nlm.nih.gov/pubmed/28122677

Evaluation of a rapid immunochromatographic test for the detection of OXA-48 carbapenemase.

Rubio E, Zboromyrska Y, Pitart C, Campo I, Alejo-Cancho I, Fasanella A, Vergara A, Marco F, Vila J.
Diagn Microbiol Infect Dis. 2017 Mar;87(3):266-267.

Abstract
We evaluated the OXA-48K-Set, a rapid immunochromatographic test for the detection of Oxacillinase-48 (OXA-48) carbapenemases, among 37 strains expressing OXA-48 and OXA-48-like carbapenemases and 20 additional strains harboring other β-lactamases. The test showed 100% sensitivity and specificity and the results were obtained in 15minutes.

https://www.ncbi.nlm.nih.gov/pubmed/27988171

Comparison of phenotypic tests and an immunochromatographic assay and development of a new algorithm for OXA-48-like detection.

Koroska F, Göttig S, Kaase M, Steinmann J, Gatermann S, Sommer J, Wille T, Plum G1, Hamprecht A.
J Clin Microbiol. 2016 Dec 28. pii: JCM.01929-16. doi: 10.1128/JCM.01929-16. [Epub ahead of print]

Abstract
OXA-48 is the most prevalent carbapenemase in Enterobacteriaceae in Europe and the Middle East, but is frequently missed because many isolates display low minimal inhibitory concentrations (MIC) for carbapenems. Furthermore, in contrast to metallo-β-lactamases or Klebsiella pneumoniae carbapenemases (KPC), no specific inhibitor is available for the phenotypic detection of OXA-48. Molecular detection of blaOXA-48 is the gold standard, but is not available in many laboratories. Few phenotypic assays have been described, but have not been independently evaluated. The aim of this study was the systematic comparison of phenotypic tests and an immunochromatographic assay (ICT) for the detection of OXA-48/OXA-48-like carbapenemases and the development of an algorithm for reliable phenotypic detection of OXA-48.Four phenotypic tests (temocillin disk test, faropenem disk test, OXA-48 disk test and HI OXA-48 disk test) and a new ICT (OXA-48 K-SeT) were compared on a set of 166 Enterobacteriaceae isolates including OXA-48/OXA-48-like (n=84), Ambler class A and B carbapenemases (n=41), and carbapenemase-negative isolates (n=41).The sensitivity and specificity for the different assays was 100%/43.9% for temocillin, 57.1%/98.8% for faropenem, 53.6%/100% for the OXA-48 disk test, 98.8%/97.6% for the HI OXA-48 disk test and 100%/100% for the ICT, respectively.The ICT displayed the highest sensitivity and specificity, was the most rapid assay but is more costly than phenotypic assays. Based on these results, a new algorithm incorporating temocillin, faropenem and ICT was developed, which allows a cost-effective detection of OXA-48 with 100% sensitivity and specificity.

https://www.ncbi.nlm.nih.gov/pubmed/28031433

Evaluation of the K-SeT R.E.S.I.S.T. immunochromatographic assay for the rapid detecion of KPC and OXA-48-like carbapenemase.

Danièle Meunier, Anna Vickers, Rachel Pike, Robert L.Hill, Neil Woodford, Katie L. Hopkins.
J Antimicrob Chemother. 2016 April 26 doi:10.1093/jac/dkw113

J. Antimicrob. Chemother.-2016-Meunier-jac-dkw113

EP0234 - Evaluation Comparison of a novel OXA-48 K-Set test and blue-carba test in detection of carbapenemase-producing enterobacteriaceae with using PCR as reference method.

A. Abulaila, F. Erdem, Z. Aktas and O. Oncul
26th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 09 – 12, 2016
Poster234

P0946 - The Coris BioConcept OXA48 K-SeT Immuno-Chromatographic Assay Detects OXA48-type Carbapenemases with High Sensitivity and Specificity.

B.M. Willey, X. Trimi, R. Ioboni, D.A. Boyd, G. Ricci, D.N. Grohn, D. Terenzi, A. Mazzulli, L. Mataseje, M. Mulvey, P. Lo, T. Mazzulli, S.M. Poutanen
26th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 09 – 12, 2016
Poster946

EV0432 - Evaluation of the performance of OXA-48 K-SeT immunochromatographic test for rapid identification of OXA-48 carbapenemase-producing Enterobacteriaceae.

O. Karatuna, M. Kaya, I. Akyar
26th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 09 – 12, 2016
Poster432

EV0445 - OXA-370 is rapidly detected from different culture media using OXA-48 K-SeT® immunochromatography.

C.S. Nodari, A. Barth, C. Magagnin, A. Zavascki, A. Gales, C. Carvalhaes
26th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 09 – 12, 2016
Poster445

P0840 - Evaluation of a rapid diagnostic test for the detection of OXA-48 carbapenemase

E. Rubio, Y. Zboromyrska, C. Pitart, I. Alejo-Cancho, I. Campo, A. Fasanella1, F. M. Reverte, J. Vila.
26th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 09 – 12, 2016
Poster840

EV0447 - A rapid test for detection of OXA-48 carbapenemase.

I. De Toro Peinado, MªC.M. Gradolph, R. S. Rodriguez, M. V. Troya, MªP. B. Ruiz, B. Palop
26th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 09 – 12, 2016
Poster447

EV0186 - Preliminay study of the OXA-48 card letitest method for the direct detection of OXA-48 carbapenemase in blood and plates culture.

A. Sarria, R. Gomez-Gil, G. Ruiz, MP. Romero, J. García- Rodríguez
26th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 09 – 12, 2016
Poster186

EV0462 – Specificity of the OXA-48 immunochromatographic K-SeT for the detection of OXA-48 like in Shewanella spp.

P. Bogaerts, S. Evrard, G. Cuzon, TD. Huang, T. Naas and Y. Glupczynski
26th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 09 – 12, 2016
Poster462

P1022 – Impact of the isolation medium for the detection of OXA-48 and KPC-producing Gram negative bacteria by immunochromatographic assays.

P. Bogaerts, S. Evrard, M. Dozen, TD. Huang and Y. Glupczynski
26th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 09 – 12, 2016
Poster1022

P0952 - Prospective evaluation of the OXA-48 K-SeT for the detection of OXA-48-type carbapenemase producing Enterobacteriaceae.

L. Dortet, A. Jousset, V. Sainte-Rose, G. Cuzon, and T. Naas
26th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 09 – 12, 2016
Poster186

Evaluation of OXA-48 K-SeT: an immunochromatographic assay for rapid detection of OXA-48-producing enteriobacteriaceae. 

Fernandez J, Fleites A, Rodcio MR, Vazquez F.
Diagn Micribiol Infect Dis. 2016 Jan 26. pii: S0732-8893(16)00034-1. doi: 10.1016/j.diagmicrobio.2016.01.015. [Epub ahead of print].

http://www.ncbi.nlm.nih.gov/pubmed/26971639

Prospective evaluation of the OXA-48 K-SeT assay, an immunochromatographic test for the rapid detection of OXA-48-type carbapenemases. 

Dortet L, Jousset A, Sainte-Rose V, Cuzon G, Naas T.
J Antimicrob Chemother. 2016 Mar 10. pii: [Epub ahead of print].

http://www.ncbi.nlm.nih.gov/pubmed/26968882

Evaluation du kit OXA-48 K-Set (CORIS BioConcept) pour la détection des souches d’entérobactéries productrices de carbapénémases de type OXA-48 isolées en Algérie. 

N.Aggoune, H.Tali-Maamar, B.Guettou , A.Zerouki , M.Naim, K.Rahal.
35e Réunion interdisciplinaire de chimiothérapie anti-infectieuse 13 - 15 décembre, 2015 Paris.
Paris 2015 - Evaluation OXA-48

Evaluation of two new commercial immunochromatographic assays for the rapid detection of OXA-48 and KPC carbapenemases from cultured bacteria. 

Glupczynski Y, Evrard S, Ote I, Mertens P, Huang TD, Leclipteux T, Bogaerts P.
J Antimicrob Chemother. 2016 Jan 28. pii: dkv472. [Epub ahead of print]

https://pubmed.ncbi.nlm.nih.gov/26825120/

Abstract :
BACKGROUND:
Rapid detection and confirmation of carbapenemases remains very challenging for diagnostic laboratories.
OBJECTIVES:
The objective of this study was to assess the performance of two new immunochromatographic (IC) commercial assays for the rapid detection of OXA-48-producing and KPC-producing Enterobacteriaceae in pure bacterial isolates.
METHODS:
A panel of 92 bacterial isolates predominantly including carbapenem-non-susceptible Enterobacteriaceae with previously defined carbapenem resistance mechanisms was tested. Then, 342 consecutive carbapenem-non-susceptible Enterobacteriaceae isolates referred to the reference laboratory were investigated prospectively in parallel with other phenotypic tests and with multiplex PCR and sequencing as the gold standard.
RESULTS:
In the collection panel, each of the two IC assays correctly detected all 30 OXA-48-like-producing isolates and 25 KPC-producing isolates, whatever the species, their association with other β-lactamases and the level of resistance to carbapenems. All other carbapenemase producers and all non-carbapenemase-producing isolates yielded negative results with both tests. In the prospective evaluation, all OXA-48-like-producing Enterobacteriaceae isolates (n = 130) and KPC-producing Enterobacteriaceae isolates (n = 33) were correctly detected by the individual IC assays, while 179 non-OXA-48-like-producing and non-KPC-producing strains (137 non-carbapenemase producers and 42 isolates belonging to other carbapenemase family types) yielded negative results. Thus, each assay yielded 100% sensitivity and 100% specificity for the detection of OXA-48-like or KPC enzymes, respectively, at 15 min.
CONCLUSIONS:
The two IC assays allow rapid and reliable direct confirmation of OXA-48 and KPC carbapenemases from culture colonies and appear to be very useful additions to the existing tests, obviating the need for more costly characterization by molecular amplification methods.

Prospective evaluation of the OXA-48 K-SeT for the detection of OXA-48-type carbapenemase producing Enterobacteriaceae. 

Laurent Dortet, Agnès Jousset, Vincent Sainte-Rose, Gaëlle Cuzon and Thierry Naas.
55th ICAAC 2015 September 17-21, 2015  San Diego, CA.
ICAAC - 2015

Evaluation of an Immunochromatographic Lateral Flow Assay (OXA-48 K-SeT) for the Rapid Detection of OXA-48-like Carbapenemases in Enterobacteriaceae. 

Wareham DW, Shah R, Betts JW, Phee LM, Abdul Momin MH.
J Clin Microbiol. 2015 Nov 25. pii: JCM.02900-15.

http://www.ncbi.nlm.nih.gov/pubmed/26607983

Abstract : 
We evaluated an immunochromatographic lateral flow assay for the detection of OXA-48-like carbapenemases (OXA-48 K-SeT) in Enterobacteriaceae (n=82). 100% sensitivity and specificity was observed using bacteria recovered from both solid media and spiked blood culture bottles, with the result obtained in less than 10 minutes.

Development of a novel immunochromatographic combo test for rapid identification of OXA-­48 and KPC carbapenemases in Enterobacteriaceae. 

Céline Borlon, Laurence Denorme, Stéphanie Evrard, Isabelle Ote, Pierre Bogaerts, Youri Glupczynski, Pascal Mertens.
115th American Society for Microbiology May 30 - June 2, 2015 New Orleans.
ASM 2015

Development of a novel immunochromatographic confirmatory test for the detection of OXA-­48 carbapenemase in Enterobacteriaceae.

Isabelle Ote, Pierre Bogaerts, Laurence Denorme, Céline Borlon, Caroline Thunissen, Youri Glupczynski, Thierry Leclipteux, Pascal Mertens.
25th European Congress of Clinical Microbiology and Infectious Diseases April-25 - 28, 2015
Development of a novel...
ECCMID presentation - Isabelle OTE

Validation of a new lateral flow assay for the detection of OXA-48-like-producing Enterobacteriaceae

Pierre Bogaerts
25th European Congress of Clinical Microbiology and Infectious Diseases April-25 - 28, 2015
ECCMID 2015
http://www.eccmidlive.org/