Our technology

Immunochromatography

Immunochromatography test (ICT) is a membrane-based immunological technology. It is used to detect specific agents in a biological sample. To this end, capture antibodies directed against the targeted pathogen are coated on a nitrocellulose membrane. A second antibody is conjugated to colloidal gold nanoparticle or colored latex beads, and dried on the conjugate pad zone of the membrane.

Immunochromatography test is a ready-to-use assay. The biological sample is first diluted in a provided dilution buffer before being dispensed on the conjugate pad zone of the membrane. Once loaded, it rehydrates the dried conjugated antibody. If the sample contains the targeted antigens, rehydrated conjugated antibody and antigen interact each other. The resulting complex then migrates on the nitrocellulose membrane by lateral flow capillarity to reach the test line where it remains bound to the first antibody adsorbed onto the nitrocellulose. A colored line appears on the test line. Solution continues to migrate to encounter the control line where a second immunoreaction happens to validate the proper migration of the test. Result is visible within 5, 10 or 15 minutes, depending on the test. Please refer to the provided instruction for use (IFU) for a complete and precise procedure

Rapid Electrochemical Detection

The BL-RED test is based on the use of an electroactive substrate, comparable to a 3GC whose enzymatic hydrolysis releases a product capable of generating an anodic oxidation current measurable by voltammetry. The intensity of such a current can be measured using screen-printed electrodes and a suitable reader configured for this use. In the BL-RED test, the measurement of a current with a greater intensity than a threshold value is the reflection of a bacterial hydrolysis activity of 3GCs, responsible for a reduced sensitivity of bacteria to these antibiotics.

Reverse Transcription Loop-mediated isothermal AMPlification (RT-LAMP)

The RT-LAMP (Reverse Transcription Loop-mediated isothermal AMPlification) assay is based on a fluorometric detection using a DNA intercalating agent. Reverse transcription and amplification are carried out in the same tube during a one-step assay.

The RT-LAMP assay is performed at 63°C for 20 minutes in a real-time thermocycler or in an equipment able to detect fluorescence of the intercalating dye while keeping the reaction tubes at 63°C.