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RESIST-BC - Science

Application and clinical impact of the RESIST-4 O.K.N.V. rapid diagnostic test for carbapenemase detection in blood cultures and clinical samples

Roth S, Berger FK, Link A, Nimmesgern A, Lepper PM, Murawski N, Bittenbring JT, Becker SL.
Eur J Clin Microbiol Infect Dis. 2021 Feb;40(2):423-428

Abstract

Invasive infections caused by carbapenemase-producing bacteria are associated with excess mortality. We applied a rapid diagnostic test (RDT) on clinical samples with an elevated likelihood of carbapenemase-producing bacteria and documented its impact on antibiotic treatment decisions. Among 38 patients, twelve tested positive for infections caused by carbapenemase-producing bacteria (31.6%), mainly in blood cultures. KPC (n = 10) was more frequent than OXA-48 (n = 2). RDT-based carbapenemase detection led to a treatment modification to ceftazidime/avibactam-containing regimens in all patients before detailed antibiotic testing results became available. Eleven patients (92%) survived the acute infection, whereas one patient with a ceftazidime/avibactam- and colistin-resistant OXA-48-positive isolate died.

Direct detection of carbapenem resistance determinants in clinical specimens using immunochromatographic lateral flow devices.

Wareham DW, Phee LM, Abdul Momin MHF.
J Antimicrob Chemother. 2018 Jul 1;73(7):1997-1998

No abstract available

Rapid detection of NDM, KPC and OXA-48 carbapenemases directly from positive blood cultures using a new multiplex immunochromatographic assay.

Hamprecht A, Vehreschild JJ, Seifert H, Saleh A.
PLoS One. 2018 Sep 14;13(9):e0204157.

Abstract

Bloodstream infections caused by carbapenemase-producing Enterobacteriaceae (CPE) are associated with treatment failure and increased mortality. Detection of CPE from blood cultures (BC) by standard methods takes 16-72 hours, which can delay the initiation of appropriate antimicrobial therapy and compromise patient outcome. In the present study, we developed and evaluated a new method for the rapid detection of carbapenemases directly from positive BC using a new multiplex immunochromatographic test (ICT). The new ICT was assessed using 170 molecularly characterized Enterobacteriaceae clinical isolates including 126 CPE (OXA-48-like (N = 79), KPC (N = 18) and NDM (N = 29)). After spiking with bacteria and incubation in a BC system, blood from positive BC bottles was hemolyzed, bacteria concentrated by centrifugation and lysed. The lysate was transferred to the RESIST-3 O.K.N. ICT (Coris BioConcept, Gembloux, Belgium), which detects OXA-48-like, KPC and NDM carbapenemases. The final results of the ICT were read when they became positive, at the latest after 15 min. All CPE isolates (126/126) were correctly detected with the new protocol (100% sensitivity, 100% specificity). There was perfect concordance between ICT results and molecular characterization. Total time to result was 20-45 min.

P0810- Rapid detection of carbapenemase-producing Enterobactyeriaceae directly from positive blood cultures by a new immunochromatographic assay

A. Hamprecht, H. Seifert and A. Saleh
28th European Congress of Clinical Microbiology and Infectious Diseases, April 21 - 24 April 2018
Poster

P2325- Rapid detection of carbapenemase-producing organisms directly from positive blood cultures using a combined MALDI-TOF MS and RESIST O.K.N. workflow

E. Wey, L. Ainsworth, T. McHugh and I. Balakrishnan
28th European Congress of Clinical Microbiology and Infectious Diseases, April 21 - 24 April 2018
Poster

Detection of OXA-370 directly from rectal swabs and blood culture vials using an immunochromatographic assay.

Nodari CS, Gales AC, Barth AL, Magagnin CM, Zavascki AP, Carvalhaes CG.
J Microbiol Methods. 2017 Aug;139:92-94

Abstract

We evaluated the performance of OXA-48 K-SeT assay for detecting OXA-370 directly from spiked rectal swabs and blood culture vials. The limit of detection of this test was 104UFC/mL for rectal swabs. Detection of the OXA-370-producing isolates was successfully achieved directly from positive blood culture vials independent of growing conditions.

The prevalence of Extended-Spectrum B-lactamse (ESBL) and carbapenem-resistant Enterobacteriaceae (CRE) isolates in positive blood cultures of patients in a teaching hospital in Kuwait over a 2-year period.

Taqi M, Jamal W and Rotimi V
Open Forum Infectious Diseases, Volume 4, Issue suppl_1, 2017, S1944

Abstract

Invasive infections caused by carbapenemase-producing bacteria are associated with excess mortality. We applied a rapid diagnostic test (RDT) on clinical samples with an elevated likelihood of carbapenemase-producing bacteria and documented its impact on antibiotic treatment decisions. Among 38 patients, twelve tested positive for infections caused by carbapenemase-producing bacteria (31.6%), mainly in blood cultures. KPC (n = 10) was more frequent than OXA-48 (n = 2). RDT-based carbapenemase detection led to a treatment modification to ceftazidime/avibactam-containing regimens in all patients before detailed antibiotic testing results became available. Eleven patients (92%) survived the acute infection, whereas one patient with a ceftazidime/avibactam- and colistin-resistant OXA-48-positive isolate died.
https://academic.oup.com › ofid › S564 › ofx163.1476.pdf