Potron A, Daniel M, Bay M, Choulet P, Garrigos T, Sababadichetty L, Belmonte O, Fournier D, Jeannot K, Miltgen G.
Microbiol Spectr. 2024 Aug 20:e0104424.doi: 10.1128/spectrum.01044-24.
Abstract
Carbapenem-resistant Acinetobacter baumannii (CRAB) are increasingly reported worldwide and a leading cause of mortality associated with antimicrobial resistance. Their early detection, particularly in the cases of bloodstream infections, is crucial in attempting to initiate effective antibiotic treatment. The immunochromatographic assay RESIST ACINETO (Coris BioConcept) is a new test developed for the detection of OXA-23, OXA-40/58, and New-Delhi Metallo-beta-lactamase (NDM) carbapenemases in Acinetobacter spp. We evaluated this test on a collection of 121 Acinetobacter spp. clinical isolates, including 104 carbapenemase producers (97 carbapenemases targeted by the test) and 17 non-carbapenemase producers. The performance of the RESIST ACINETO test was evaluated according to the manufacturer's recommendations from bacterial and blood cultures. The strains producing the carbapenemases OXA-23, -40, -58, or/and NDM were accurately detected from bacterial cultures and directly from blood cultures, with the exception of one OXA-23/NDM-1-positive Acinetobacter radioresistens isolate (only detected through standard culture). None of the non-carbapenemase producers tested positive. The RESIST ACINETO test demonstrated sensitivity/specificity of 100%/100% and 99%/100% on bacterial and blood cultures, respectively.
A. Potron, M. Daniel, P. Triponney, T. Garrigos, L. Sababadichetty, K. Jeannot, O. Belmonte, D. Fournier, G. Miltgen
43e Réunion interdisciplinaire de chimiothérapie anti-infectieuse 18 - 19 décembre, 2023 Paris.
Bouvier M, Kerbol A, J Findlay, Freire S, Poirel L and Nordmann P
Diagnostic Microbiology and Infectious Disease 107 (2023) 116043
Abstract
The Resist Acineto from Coris Bioconcept is a novel immunochromatographic test for detection of the major acquired carbapenemases (OXA-23, OXA-40, OXA-58, and NDM) identified in Acinetobacter spp. This rapid and easy-to-perform test showed an excellent specificity and sensitivity, with positive and negatives predic tive values of 100% in both cases
Bouvier M, Kerbol A, J Findlay, Freire S, Poirel L and Nordmann P
13th Symposium on the Biology of Acinetobacter 21st – Coimbra - 2023
Mertins S, Higgins PG, Thunissen C, Magein H, Gilleman Q, Mertens P, Rodríguez MG, Maus LM, Seifert H, Krönke M, Klimka A.
J Med Microbiol. 2023 Apr;72(4).
Abstract
Introduction. Acinetobacter baumannii infections can be extremely challenging to treat owing to the worldwide prevalence of multidrug-resistant isolates, especially against carbapenems. Colonization with carbapenem-resistant A. baumannii (CRAb) requires rapid action from an infection control perspective because the organism is known for its propensity for epidemic spread. Hypothesis/Gap Statement. There is an unmet medical need to rapidly identify CRAb to enable appropriate antimicrobial treat ment and to prevent transmission. Aim. Our aim was to expand the OXA-detection abilities of the rapid immunochromatographic test (ICT) OXA-23 K-SeT (Coris BioConcept) to include OXA-40- and OXA-58-like carbapenemases, which together confer carbapenem resistance to more than 94% of CRAb isolates worldwide.
Methodology. We used hybridoma technology to generate mAbs against OXA-40 and OXA-58 and selected them for productivity and specificity against recombinant and endogenous OXA-40 and OXA-58. Combinations of the resulting mAbs were analysed in ICT format for their ability to detect recombinant rOXA-40His6 or rOXA-58His6, respectively. Subsequently, selected antibody pairs were implemented into single-OXA-40 or single-OXA-58 prototypes and the final OXA-23/40/58/NDM ICT and were evaluated on clinical Acinetobacter spp. isolates with well-defined carbapenem resistance mechanisms.
Results. Five anti-OXA-40 and anti-OXA-58 mAbs were selected. Competition ELISA with combinations of these antibodies revealed that the anti-OXA-40 antibodies bind to one of two binding clusters on OXA-40, while anti-OXA-58 antibodies bind to one of four binding clusters on OXA-58. Direct binding to the corresponding antigen in an ICT format has left only three anti bodies against rOXA-40His6 and rOXA-58His6, respectively for the subsequent sandwich ICT selection procedure, which revealed that the anti-OXA-40 (#5) and anti-OXA-58 (#A8) mAbs in combination with the cross-reactive mAb #C8 performed best. They were implemented into single-OXA-40 and single-OXA-58 ICT prototypes and evaluated. These single ICT prototypes demon strated 100% specificity and sensitivity. Based on these results, an OXA-23/40/58/NDM-ICT was developed, complemented with OXA-23 and NDM-specific detection. An evaluation with selected carbapenem-resistant Acinetobacter spp. isolates (n=34) showed 100% specificity.
Conclusion. With this easy-to-use detection assay, one can save 12–48 h in diagnostics, which helps to treat patients earlier with appropriate antibiotics and allows immediate intervention to control transmission of CRAb.
Mancini S, Seth-Smith HMB, Kolesnik-Goldmann N, Hinic V, Roloff T, Imkamp F, Egli A.
J Antimicrob Chemother. 2023 Nov 6;78(11):2771-2774
Abstract
No abstract available
CREALYS Science Park
Rue Guillaume Fouquet 11
5032 Gembloux - BELGIUM
Ph.: +32 (0)81 719 917
Administration
info@corisbio.com
Sales
Sales@corisbio.com
Client Care
client.care@corisbio.com
Copyright © 2025 Coris BioConcept All rights reserved - Privacy Policy - Site Map - FAQ - Cookie manager
