Coris > Products > RESIST-3 O.K.N. K-SeT > Science
Ebongue CO, Simo GG, Mefo'o JP, Ngondi GD, Mengue ER, Ngaba GP and Adiogo D.
Advances in Microbiology, 2021, 11, 579-590
Abstract
Background: The increasing resistance of bacteria to various antibiotics is a worldwide public health issue. Carbapenems that have elicited great hope in treating infections caused by multidrug-resistant germs have seen their efficacy narrowed over time with the emergence of other novel resistance mechanisms, notably the production of Carbapenemases. Methods: A prospective cross-sectional study was conducted from May 2017 to May 2018 in Douala (Cameroon) to detect carbapenemase-producing Gram-negative bacilli. Isolated strains were identified using the Vitek2TM system. Antimicrobial susceptibility testing was performed using the Kirby-Bauer disk diffusion method on agar plates with 20 selected commercially available antibiotic discs. The bacterial strains were tested for the production of three Carbapenemases (OXA-48, NDM, KPC), using an immuno-chromatographic technique, with the “RESIST-3 O.K.N. K-SeT” rapid detection kit. Results: During the study period, 1687 strains of Gram-negative bacilli were isolated in selected laboratories with a total of 200 multi-resistant strains identified (11.9%). Among the multi-resistant strains, E. coli was the species most represented in Enterobacteriaceae (27.5%) followed by K. pneumoniae (15.5%) and the non-fermenting Gram-negative bacilli were predominantly P. aeruginosa (20.5%). These strains mainly came from urine and pus, i.e. 41% and 32% respectively. Thirty-two (16%) strains produced one of the Carbapenemases with a higher frequency at the General Hospital (84%). NDM-type carbapenemase was the most frequently identified (8.5%), OXA-48 type 7.5%, and no KPC production was observed. Among the Enterobacteriaceae 22.9% produced Carbapenemases and only 5.1% of the non-fermenting bacilli produced these enzymes. The isolates strains were completely resistant to all antibiotics except Amikacin and Fosfomycin. The strains producing the NDM-type carbapenemase showed higher rates of resistance to almost all of the antibiotics tested. Conclusion: Multidrug-resistant strains are experiencing an increase in evolution. The apparition of strains producing Carbapenemases prominently, the NDM and OXA-48 favor this increase. The activities of antibiotics with high efficacies on these strains are low.
https://www.scirp.org/journal/paperinformation.aspx?paperid=112701
Pitout JDD, Peirano G, Kock MM, Strydom KA, Matsumura Y
Clin Microbiol Rev. 2019 Nov 13;33(1):e00102-19
Abstract
Surveillance studies have shown that OXA-48-like carbapenemases are the most common carbapenemases in Enterobacterales in certain regions of the world and are being introduced on a regular basis into regions of nonendemicity, where they are responsible for nosocomial outbreaks. OXA-48, OXA-181, OXA-232, OXA-204, OXA-162, and OXA-244, in that order, are the most common enzymes identified among the OXA-48-like carbapenemase group. OXA-48 is associated with different Tn1999 variants on IncL plasmids and is endemic in North Africa and the Middle East. OXA-162 and OXA-244 are derivatives of OXA-48 and are present in Europe. OXA-181 and OXA-232 are associated with ISEcp1, Tn2013 on ColE2, and IncX3 types of plasmids and are endemic in the Indian subcontinent (e.g., India, Bangladesh, Pakistan, and Sri Lanka) and certain sub-Saharan African countries. Overall, clonal dissemination plays a minor role in the spread of OXA-48-like carbapenemases, but certain high-risk clones (e.g., Klebsiella pneumoniae sequence type 147 [ST147], ST307, ST15, and ST14 and Escherichia coli ST38 and ST410) have been associated with the global dispersion of OXA-48, OXA-181, OXA-232, and OXA-204. Chromosomal integration of bla OXA-48 within Tn6237 occurred among E. coli ST38 isolates, especially in the United Kingdom. The detection of Enterobacterales with OXA-48-like enzymes using phenotypic methods has improved recently but remains challenging for clinical laboratories in regions of nonendemicity. Identification of the specific type of OXA-48-like enzyme requires sequencing of the corresponding genes. Bacteria (especially K. pneumoniae and E. coli) with bla OXA-48, bla OXA-181, and bla OXA-232 are emerging in different parts of the world and are most likely underreported due to problems with the laboratory detection of these enzymes. The medical community should be aware of the looming threat that is posed by bacteria with OXA-48-like carbapenemases.
https://pubmed.ncbi.nlm.nih.gov/31722889/
Wink PL, Martins AS, Inamine E, Dalmolin TV, Barth AL.
Braz J Microbiol. 2019 Jul;50(3):657-662
Abstract
The emergence of carbapenem-resistant Enterobacterales (CRE) is a matter of public health concern. Carbapenemases are the main mechanism of resistance among CRE, and its rapid detection is essential. The detection of carbapenemases usually requires culture-based methods and molecular assays, which may be costly and need long turnaround times. Recently, an easy and rapid immunochromatographic assay for carbapenemases (OXA-48, KPC, and NDM) detection based in lateral flow immunoassay with specific monoclonal antibodies on a nitrocellulose membrane has been developed. We aimed to evaluate the RESIST-3 O.K.N. in colonies from pure culture as well as in spiked blood cultures with Enterobacterales. All carbapenemase producers (CP) presenting the OXA-48-like, KPC, and NDM enzymes presented positive results in both pure colonies and spiked blood cultures. None of the carbapenemase non-producers (CNP) presented positive results in the tests. A total of 97% CP isolates presented positive results in pure colonies in less than 5 min. For CP directly from blood culture, the mean time to positivity for OXA-48-like and KPC was 1 min, whereas it was 25 min for NDM. Our results indicate that this immunoassay can be used to detect carbapenemases directly from blood culture bottles in a routine diagnostic laboratory, which would reduce the turnaround time of CP detection.
https://www.ncbi.nlm.nih.gov/pubmed/31270693
Hamprecht A, Vehreschild JJ, Seifert H, Saleh A.
PLoS One. 2018 Sep 14;13(9):e0204157
Abstract
Bloodstream infections caused by carbapenemase-producing Enterobacteriaceae (CPE) are associated with treatment failure and increased mortality. Detection of CPE from blood cultures (BC) by standard methods takes 16-72 hours, which can delay the initiation of appropriate antimicrobial therapy and compromise patient outcome. In the present study, we developed and evaluated a new method for the rapid detection of carbapenemases directly from positive BC using a new multiplex immunochromatographic test (ICT). The new ICT was assessed using 170 molecularly characterized Enterobacteriaceae clinical isolates including 126 CPE (OXA-48-like (N = 79), KPC (N = 18) and NDM (N = 29)). After spiking with bacteria and incubation in a BC system, blood from positive BC bottles was hemolyzed, bacteria concentrated by centrifugation and lysed. The lysate was transferred to the RESIST-3 O.K.N. ICT (Coris BioConcept, Gembloux, Belgium), which detects OXA-48-like, KPC and NDM carbapenemases. The final results of the ICT were read when they became positive, at the latest after 15 min. All CPE isolates (126/126) were correctly detected with the new protocol (100% sensitivity, 100% specificity). There was perfect concordance between ICT results and molecular characterization. Total time to result was 20-45 min.
CONCLUSIONS: This proof-of-principle study demonstrates that with the newly developed method, OXA-48-like, KPC and NDM carbapenemases can be reliably detected directly from positive BC bottles. The new method is more rapid than other currently available assays and can be performed in any routine microbiology laboratory. This can help to rapidly identify patients with CPE BSI and optimize the management of patients with these difficult-to-treat infections. Further studies are needed to assess the performance of the ICT in routine diagnostics.
https://www.ncbi.nlm.nih.gov/pubmed/30216371
NADJI S, MESSAADI S, BERTO E, CALOONE C, SZCZAPA V, LOUKILI N, LEMAITRE N.
38e Réunion interdisciplinaire de chimiothérapie anti-infectieuse 17-18 décembre, 2018 Paris.
Poster
Fauconnier C, Dodemont M, Depouhon A, Anantharajah A, Verroken A, Rodriguez-Villalobos H.
J Antimicrob Chemother. 2018 Oct 30
Abstract
Background:
Rapid and effective screening of carbapenemase-producing Enterobacteriaceae (CPE) appears essential for adequate patient management and rapid implementation of infection control measures. Most of these screening techniques require a minimum of 24 h of culture. Molecular assays are an exception since these can be achieved within 1 h, but are expensive and usually require specialized facilities and trained personnel. In this context, lateral immunochromatography performed directly from rectal swab samples could represent a cost-effective alternative with a reduced turnaround time.
Objectives: In this study, we assessed the performance of the OKN K-SeT test (Coris BioConcept, Gembloux, Belgium) for the rapid detection of OXA-48, KPC and NDM CPE directly from rectal swab samples.
Methods: A total of 149 residual rectal swabs, routinely screened for CPE through selective culture and confirmed by PCR, were tested with a defined protocol consisting of a 2.5 h incubation of the swab in an enrichment medium containing meropenem followed by OKN K-SeT testing after centrifugation.
Results:This method displayed an overall sensitivity of 96% and a specificity of 100% with a limit of detection ranging between 104 and 105 cfu/mL.
Conclusions: Whereas this assay appears highly specific, it displays a reduced sensitivity compared with the standard procedure encompassing a culture step. Nonetheless, this rapid method allows an accelerated identification of most CPE carriers at a lower cost and, accordingly, the implementation of early appropriate management procedures.
https://www.ncbi.nlm.nih.gov/pubmed/30376099
Zarakolu P, Aslan AT, Perry J.
Turk J Med Sci. 2018 Jun 14;48(3):679-680. doi: 10.3906/sag-1804-23
Abstract
N.A.
https://www.ncbi.nlm.nih.gov/pubmed/29916229
D’Aleo F., Ceccarelli M., Facciolà A., Venanzi Rullo E., Paolucci I., Lo Presti Costantino M. R., Fazii P., Conte M., Pellicanò G. F.
Infectious Diseases & Tropical Medicine 2018; 4 (2): e470
Abstract
Multidrug resistance is growing at an alarming rate among a variety of bacterial species, causing infections, which threaten the lives of patients.
Detection of metal-β-lactamase producers (IMP, VIM, NDM) and of KPC producers may be based on the inhibitory properties of several molecules but requires additional expertise and time.
Several authors describe the use of new rapid identification methods. The aim of our review is to revise the new rapid methods for the detection of carbapenemases and their identification by Gram-negative bacilli.
https://www.infectiousjournal.com/article/470
M. Wilks, A. Whiley, A. Alcolea-Medina and B.P. Cherian.
28th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 21 – 24, 2018.
Poster
E. Wey, L. Ainsworth, T. McHugh and I. Balakrishnan.
28th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 21 – 24, 2018.
Poster
B.M. Willey, M. Al-Ani, Y. Sokolskyy, E. Osuji, A. Paterson, DA. Boyd, W. Chui, T. Mazzulli and S.M. Poutanen.
28th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 21 – 24, 2018.
Poster
B.M. Willey, M. Al-Ani, Y. Sokolskyy, E. Osuji, A. Paterson, DA. Boyd, W. Chui, T. Mazzulli and S.M. Poutanen.
28th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 21 – 24, 2018.
Poster
S. Tsiplakou, V. Papaioannou, E. Koiliari, D. Stefani and M. LelekisA. Saleh, S. Göttig and A. Hamprecht.
28th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 21 – 24, 2018.
Poster
A.Hamprecht, H. Seifert and A. Saleh.
28th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 21 – 24, 2018.
Poster
A. Saleh, S. Göttig and A. Hamprecht.
28th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 21 – 24, 2018.
Poster
Wareham DW, Phee LM, Abdul Momin MHF.
J Antimicrob Chemother. 2018 Mar 22. doi: 10.1093.
Abstract
n.a.
P. Sağıroğlu, U. Hasdemir, G. Altınkanat Gelmez, B. Aksu, O. Kataruna and G. Söyletir.
Brazilian Journal of Microbiology 49 (2018)885–890 Available online 1 March 2018.
Abstract
In this study, the performance of the “RESIST-3 O.K.N. K-SeT” (Coris BioConcept, Gembloux, Belgium) immunochromatographic assay was evaluated in 132 Klebsiella pneumoniae comprising 102 carbapenem resistant and 30 carbapenem susceptible isolates. Genotypically known isolates of Gram negative bacteria (n = 22) including various species were also tested by the assay as controls. The isolates tested by the immunochromatographic assay and also were run PCR for blaKPC, blaIMP, blaVIM, blaNDM, and blaOXA-48. The rates of blaNDM, blaOXA-48, and blaKPC in carbapenem resistant isolates were found at 52.9%, 39.2%, and 2.0%, respectively. Both blaNDM and blaOXA-48 were found in six (5.9%) isolates. The results of the assay showed 100% concordance with those obtained by PCR in 132 K. pneumoniae. The agreement between the two methods was found to be identical at the isolate level. The assay also correctly detected all genotypically known isolates of Escherichia coli, Serratia marcescens, Citrobacter freundii, Enterobacter cloacae, K. pneumoniae carrying blaKPC, blaNDM, and/or blaOXA-48. On the other hand, the assay did not exhibit any cross-reaction in control isolates harboring blaIMP and blaVIM. We conclude that the RESIST-3 O.K.N. K-SeT is a reliable, rapid, and user friendly test and we recommend it for routine diagnostic laboratories.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175700/pdf/main.pdf
A. Saleh, S. Göttig and A. Hamprecht.
J Clin Microbiol. 2018 Feb 14. pii: JCM.00050-18.
Abstract
SYNOPSISFor rapid detection of carbapenemase-producing Enterobacteriaceae (CPE), immunochromatographic lateral flow tests (ICT) have recently been developed. The aim of this study was to assess the new multiplex ICT RESIST-3 O.K.N®. and to investigate if it can be performed directly from susceptibility testing plates. Additionally the impact of the inoculum and carbapenem disks on sensitivity and specificity was evaluated. The new ICT was challenged using 63 CRE isolates, including 51 carbapenemase producers. It was assessed using five different conditions directly from Mueller-Hinton agar (MHA): 1 μl or 10 μl inoculum harvested in the absence of antibiotic pressure or 1 μl taken from the inhibition zone of either ertapenem, imipenem or meropenem disks.The sensitivity of the ICT was 100 % for OXA-48-like and KPC and 94.4 % for NDM with the 1 μl inoculum. When harvested adjacent to a carbapenem disk, the sensitivity increased to 100 %. Additionally, with zinc-supplemented MHA both the sensitivity increased and the NDM band became visible faster (mean time 8 ± 3.9 min for MHA compared to 1.9 ± 1.5 min for MHA+zinc, P=0.0016). The specificity of the ICT was 100%.Conclusions: The RESIST-3 O.K.N. ICT is a sensitive and rapid test for the detection of three highly prevalent carbapenemases. However, false-negative results for NDM can occur. We recommend an inoculum of 1 μl that is harvested adjacent to an ertapenem or meropenem disk and the use of agars with sufficient zinc content to achieve the best performance.
http://jcm.asm.org/content/early/2018/02/09/JCM.00050-18.long
G. L. Vanstone, S. Woodhead, K. Roulston, H. Sharma, E. Wey, E. R. Smith, D. Mack and I. Balakrishnan.
Article is presented in J Med Microbiol. 2018 Feb;67(2):208-214.
Abstract
PURPOSE:
Carbapenemase-producing organisms (CPOs) can be resistant to almost all β-lactams and represent an increasing threat in healthcare facilities. Detection of these organisms in routine diagnostic laboratories is difficult; here we evaluate four commercially available CPO detection assays and assess their suitability for the clinical laboratory.
METHODOLOGY:
A panel of 95 clinical multidrug-resistant organisms (22 NDM, 24 OXA-48, 19 VIM, 4 OXA-23, 3 KPC, 4 NDM+OXA-48, 1 OXA23+NDM, 1 IMI, 1 IMP-1, 9 ESBL, 3 derepressed AmpC and 4 inducible AmpC producers) were tested by the RESIST-3 O.K.N., RapidEC CarbaNP, Acuitas Resistome and Xpert Carba-R assays.Results/Key Findings. The commercial assays performed well, with high sensitivities (96.2-100 %) and specificities (all, 100 %). The RapidEC CarbaNP and Acuitas Resistome were able to detect the broadest range of carbapenemase genotypes. The RESIST-3 O.K.N. and Xpert CarbaR had the shortest turnaround times, whilst the RapidEC CarbaNP was the only assay included in this study that could detect previously undescribed genotypes.
CONCLUSION:
Using an algorithm of the RapidEC CarbaNP, followed by either the RESIST-3 O.K.N. (Enterobacteriaceae) or the Xpert Carba-R (Pseudomonas aeruginosa and Acinetobacter spp.) on suspect CPOs allowed rapid in-house detection and genotyping of a high proportion of CPOs, reducing turnaround time by up to 7 days.
http://jmm.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000674#tab2
S. Dos Santos, L. Mereghetti, N. Van der Mee-Marquet.
RICAI
37e Réunion interdisciplinaire de chimiothérapie anti-infectieuse 18-19 décembre, 2017 Paris
Poster
De Rosa P., Abagnale I., Fusco A., Spanò A., Filosa A.
XLVI Congresso Nazionale AMCLI 11-14 Novembre, 2017 Rimini – PALACONGRESSI
Poster
Meroni E., Mauri C., Viaggi V., Pini B., Principe L., Luzzaro F.
XLVI Congresso Nazionale AMCLI 11-14 Novembre, 2017 Rimini – PALACONGRESSI
Poster
Brossa S., Cipriani R., Zanotto E., Quaranta M.R., Morcinelli M., Zaccaria T., Cavallo R.
XLVI Congresso Nazionale AMCLI 11-14 Novembre, 2017 Rimini – PALACONGRESSI
Poster
G L Vanstone, E Wey, R Smith, D Mack and I Balakrishnan.
Article is presented on website of Pathology in Practice magazine and at the Institute of Biomedical Science congress, 2017 24-27 Sept in Birmingham, UK.
Abstract
Multidrug resistance exhibited by a range of microorganisms is a growing problem, a prime example being that resulting from carbapenemase production.
Here, Gemma Vanstone and colleagues assess the value of the RESIST-3 O.K.N. lateral-flow immunochromatography assay.
Y. Glupczynski, A. Jousset, S. Evrard, R. Bonnin, TD Huang, L. Dortet, P. Bogaerts and T. Naas.
27th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 22 – 25, 2017
Poster
Wareham DW and Momin MHFA.
J. Clin. Microbiol. Accepted manuscript posted online 1 February 2017 doi:10.1128/JCM.02471-16
Abstract
The identification, treatment and control of carbapenem resistant Enterobacteriaceae (CRE) infections are a major challenge for healthcare institutions and diagnostic laboratories worldwide.…
G. L. Vanstone, E. Wey, R. Smith, D. Mack, I. Balakrishnan
Poster
Glupczynski Y, Jousset A, Evrard S, Bonnin RA, Huang TD, Dortet L, Bogaerts P, Naas T.
J Antimicrob Chemother. 2017 Mar 22
Abstract
Objectives:
There is an urgent need for accurate and fast diagnostic tests capable of identifying carbapenemase producers. Here, we assessed the performance of a new multiplex lateral flow assay (OKN K -SeT) for the rapid detection of OXA-48-like, KPC and NDM carbapenemase-producing Enterobacteriaceae from culture colonies.
Methods:
Two hundred collection isolates with characterized β-lactamase content and 183 non-duplicate consecutive isolates referred to two National Reference Centres over a 2 month period in 2016 were used to evaluate the OKN K -SeT assay.
Results:
The assay correctly detected all 42 OXA-48-like-, 27 KPC- and 30 NDM-producing isolates from the collection panel, including 7 isolates that co-produced NDM and OXA-181 carbapenemases. No cross-reactivity was observed with non-targeted carbapenemases ( n = 41) or with non-carbapenemase producers ( n = 60). Prospectively, all OXA-48-like ( n = 69), KPC ( n = 9) and NDM ( n = 19) carbapenemase-producing Enterobacteriaceae isolates were correctly detected, while 11 carbapenemase producers not targeted by the assay went undetected [VIM ( n = 8) and OXA-23/OXA-58-like ( n = 3)]. Overall, the sensitivity and specificity of the assay were 100%.
Conclusions:
The OKN assay is efficient, rapid and easy to implement in the workflow of a clinical microbiology laboratory for the confirmation of OXA-48, NDM and KPC carbapenemases. This test represents a powerful diagnostic tool as it enables the rapid detection of the most clinically important carbapenemases without the need for more costly and less frequently available molecular assays.
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