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VETERINARY DIAGNOSTIC

Prevalence and symptoms of Rotavirus and Calicivirus Infections from porcine population in the province of Zaragoza, Spain.

Halaihel N, Masía RM, de Blas I, García J, Múzquiz JL, Buesa J, Gironés O
ASM Biodefense and Emerging Diseases 2008
Rotavirus and Calicivirus infections

New approach for veterinarian diagnostics.

Achacha M, Duhamel G, Kheyar A and Villeneuve A.
American Association of Swine Veterinarians 2005  pp 69-73

Abstract:

A new rapid immunochromarographic strip assay (lSA) was developed using specifie monoclonal antibodies against various pathogenic agents. These tests use new homogenous immunochromatographic system with gold particles and directed against specifie pro teins of

the microorganism selected. These tests were evaluated and also compared with other commercial ELISA for the diagnosis of respiratory and gastroenteritis infections in experimentally and naturally infected animaIs. The specificity and sensitivity of the ISA test was determined using different dilution of faecal and serum samples.ln this study, the ISA test which works on the saille principle as ELISA gave a highly percentage of sensitivity and specificity indicating that ISA test could be used to corroborate the results of available commercially ELISA test. These tests were found more suitable for diagnosis of porcine infections and could be used for monitoring pig infected herds. These tests were found to be simple, rapid, sensitive and specifie which could be used as an alternative to the ELISA for the screening of pig infected herds under field conditions

Evaluation of Endotoxaemia in the Prognosis and Treatment of Scouring Merino Lambs

Jimenez A, Sanchez J, Andres S, Alonso JM, Gomez L, Lopez F and Rey J.
Journal of Veterinary Medicine 2007 54(2): 103–106

Abstract:

This study looked at measurement of endotoxaemia as a tool in determining prognosis and probable response to treatment in scouring lambs. One hundred eighty-three lambs in the first 15–20 days of life, from eight Merino sheep farms located in the region of La Serena, south-west Spain, were used in this experiment. Scouring and normal/control lambs were selected following a clinical examination, the scouring group was further divided into subgroups, specifically those that did or did not survive 72 h following treatment. At the time of the clinical examination, faecal and blood samples were taken. Faecal culture and commercial faecal antigen tests for detection of enteropathogens in faeces and serum endotoxin measurement using chromogenic lymulus amoebocyte lysate (LAL) were carried out. Scouring lambs received 0.07 mg/kg liveweight halofuginone once a day for 3 days, a single oral dose of 0.20 mg/kg liveweight of spectinomycin and oral rehydration fluid. The pathogens isolated were Cryptosporidium spp. and Escherichia coli. The case fatality rate was 51% in the scouring lambs. Postmortem findings were consistent with enterotoxigenic E. coli infection. The concentration of endotoxin was 0.18 ± 0.12 ng/ml in the control group, 0.35 ± 0.17 ng/ml in the surviving lambs and 0.46 ± 0.14 ng/ml in the non-surviving lambs. Significant differences between groups were found. Case fatality rate of the scouring lambs with endotoxaemia below 0.30 ng/ml was 0%, while it was 100% above 0.50 ng/ml. These results may be utilized as a prognostic indicator in lambs affected by E. coli and Cryptosporidium that will help aid in decision-making as to whether to treat a lamb or not based on its chances of survival.

Cryptosporidium felis Infection, Spain

Llorente M, Clavel A, Varea M, Goñi MP, Sahagún J and Olivera S.
Emerging Infectious Diseases • www.cdc.gov/eid • Vol. 12, No. 9, September 2006 1471-1472.

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