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Souche A, Descours G, Ranc AG, Lina G, Jarraud S, Beraud L
Eur J Clin Microbiol Infect Dis. 2020 Sep;39(9):1641-1644
Abstract
Detection of Legionella pneumophila serogroup 1 urinary antigens is the most widely used technique for the diagnosis of Legionnaires' disease (LD). The aim of this study was to evaluate the performance of the Legionella K-set® immunochromatographic test, in comparison with the BinaxNOW® Legionella urinary antigen card (UAC) on concentrated urine samples (US). A total of 250 concentrated US including 200 prospective US sent to the laboratory for urinary antigens' testing and 50 frozen US from patients with confirmed LD were tested. Positive US were retested after boiling (5 min, 100 °C). Each US leading to discordant results between the two tests was further tested using Binax™ Legionella EIA. Then, 10 additional positive non-concentrated US were tested using both tests. On concentrated US, Legionella K-set® test showed concordant results with that of BinaxNOW® Legionella. All negative US with BinaxNOW® were negative with Legionella K-set® test. For the 50 frozen US, all were positive with BinaxNOW® and 49 were positive with Legionella K-set®, all confirmed after boiling except 3 US which led to uninterpretable results with Legionella K-set®, due to a migration defect. Three of the 10 additional positive non-concentrated US were found negative with Legionella K-set® and only 1 US remained negative after concentration. All these positive non-concentrated US were positive with BinaxNOW® Legionella. The performance of the Legionella K-set® test is comparable to that of BinaxNOW® Legionella UAC, if performed on concentrated US.
https://pubmed.ncbi.nlm.nih.gov/32303927/
A. Souche, A-G. Ranc, G. Descours, C. Ginevra, G. Lina, S. Jarraud, L. Beraud
RICAI 2016
Evaluation de Legionella K-SeT
A. Souche, A-G. Ranc, G. Descours, C. Ginevra, G. Lina, S. Jarraud, L. Beraud
ESGLI 2016
Evaluation of Legionella K-SeT
Evaluation of the Legionella V-TesT Coris
Evaluation of the Legionella V-TesT Coris
E. de Witte, K. Loens, C. Lammens, J. Almirall, H. Goossens and M. Ieven
ICAAC 2011
Evaluation of the Legionella V-TesT Coris
G. Ciarrocchi, M. D’anzeo, E. Farnocchia and M.E. Cimarelli
Microbiologia Medica 25: 195-196, 2010
Evaluation of a new rapid test for detection of Legionella
José Dominguez September 6, 2010
Evaluation of Legionella V-TesT for the detection of Legionella pneumophila antigen in urine samples.
Evaluation of the Coris legionella test
Bruin. J.P, Peeters M.F., IJzerman E.P.F, Diederen. B.M.W
ECCMID 2010
Evaluation of Legionella V-TesT
Marian Garcia Nunez
December 21, 2008
Evaluation of Legionella TesT Coris
Bruin JP, Peeters MF, Ijzerman EP, Diederen BM.
Eur J Clin Microbiol Infect Dis. 2010 Jul;29(7):899-900. Epub 2010 Apr 29.
Abstract
We evaluated a new immunochromatographic assay (Legionella V-TesT, Coris BioConcept, Gembloux, Belgium) for its ability to detect Legionella pneumophila serogroup 1 antigen in urine. Test devices were read at various time points to determine the optimum incubation time regarding performance. The results were compared with those obtained with the BinaxNOW urinary antigen test. The sensitivity and specificity were 82.2% and 98.6%, respectively, for the Legionella V-TesT and 83.9% and 100%, respectively, for the BinaxNOW urinary antigen test after 15 min of incubation. When tests were examined after 60 min, the sensitivity for both tests increased to 91.5%