ROTAVIRUS - Science
• Validation of Copan Fecal Swab for the detection of rotavirus and adenovirus from fecal specimens using the Coris Rota–Strip and Adeno–Strip rapid antigens tests
Castriciano C, Booth M and Leclipteux T,
Copan Fecal Swab vs Coris kit rota adeno
• Detectability evaluation of virus used in rotavirus vaccines by the Coris diagnostic kits.
Evaluation of RotaStrip kit
• Prevalence of Rotavirus, Adenovirus and Enteric Parasites Among Pediatric Patients Attending Saint Camille Medical Centre in Ouagadougou
Ouermi Djeneba, Karou Damintoti, Ilboudo Denise, Nadembega W. Marie Christelle, Pietra Virgilio, Belem Adrien, Simpore Jacques, Kabre Gustave, Pignatelli Salvatore and Sawadogo Laya
PakistanJournal of Biological Sciences 10 (23): 4266-4270, 2007
Prevalence of Rotavirus and Adenovirus
• Tests for the rapid diagnostic of rotavirus.
Laura Alonso García, Gloria Domínguez Ortega.
Guía_ABE_Pruebas para la detección rápida del rotavirus (v.1/2007).
Tests for the Rapid Diagnostic of Rotavirus
• The Clatterbridge Hospital Study: Comparison of One-Step Assays to the DAKO ELISA
Department of Microbiology, Clatterbridge Hospital, Wirral, England.
Coris RotaStrip and DAKO ELISA
• Evaluation of Seven Commercial Assays for Detecting Group A Rotavirus Antigen.
PHLS East Virology Advisory Committee – 2001.
Evaluation of 7 assays Rotavirus
• Primary care-based surveillance to estimate the burden of Rotavirus gastroenteritis in children aged <5 years in Europe
Meyer N, Diez-Domingo J, Baldo JM, Giaquinto C, Pazdiora P, Forster J, Patrzalek M, Salter R, Pirçon Jy, Barberousse C, SOriano- Gabarró M.
ESPID, May 13-17, 2008, Graz-Austria
• Set-up of a New Rapid Immunochromatographic Diagnostic for Rotavirus Detection.
D. Van Beers , M. De Foor , R. Viehoff , D. Col , M. Venuti and Th. Leclipteux.
Progress in Clinical Virology III, Bologne, September 1997.
• Comparison of Three Rapid Immunoassays for the Detection of Rotavirus Antigen in Stool Samples.
I. Van der Donck, A. Lemmens, F. Struyf, and M. Van Ranst.
ESCV Winter Meeting 1999, Rotterdam, The Netherlands, p. 46.
Comparison of 3 assays Rotavirus
Evaluacion de 3 metodos Rotavirus
• Uso del tampone rettale come metodo per l’identificazione precoce dei portatori asintomatici di Rotavirus
Gianino P, Mastretta E, Longo P,Tubino D, Grosso F, Peltran A and Musso A.
Med Lab, Vol. 7, N. 3, 1999 P440.
• Evaluacion de trs métodos de deteccion de Rotavirus en Héces
Wilhelmi I, Roman E, Dalton R, Cubero E, Cuétara P, Sánchez-Fauquier A.
VI CONGRESO NACIONAL DE VIROLOGÍA 26th-27th 1999 - Madrid
• New Immunochromatographic Method for Rapid Detection of Rotaviruses in Stool Samples Compared with Standard Enzyme Immunoassay and Latex Agglutination Techniques
I. Wilhelmi · J. Colomina · D. Martín-Rodrigo E. Roman · A. Sánchez-Fauquier
Eur J Clin Microbiol Infect Dis. 2001 Oct;20(10):741-3.
Three different commercial immunologic tests for rapid detection of group A rotavirus (an immunochromatographic method, latex agglutination, and enzyme immunoassay) were used to evaluate 228 faecal specimens obtained from Spanish children with acute gastroenteritis. After resolution of 30 (13.2%) discordant results by reverse transcription-polymerase chain reaction
for rotavirus, the statistical values of the enzyme immunoassay, latex agglutination, and immunochromatographic method were respectively 96%, 68%, and 99% for sensitivity; 99%, 99%, and 96% for specificity; 98%, 96%, and 92% for positive predictive value; and 98%, 88%, and 99% for negative predictive value. The immunochromatographic technique showed high sensitivity and specificity and was rapid and easy to perform in the routine clinical laboratory.
• Rapid detection of rotaviruses--are laboratories underestimating infection in infants?
Dewar J, de Beer M, Elliott E, Monaisa P, Semenya D, Steele A.
S Afr Med J. 2005 Jul;95(7):494-5.
• Evaluation of seven immunochromatographic assays for the rapid detection of human rotaviruses in fecal specimens
F. Bon, J. Kaplona, M.-H. Metzgerb, P. Pothiera,*
Pathologie Biologie 55 (2007) 149–153.
Seven commercially available immunochromatographic assays were tested for the rapid detection of group A rotaviruses in fecal samples compared to a enzyme immunoassay (Argene). Detection of rotaviruses in 80 ELISA positive frozen stool samples showed rates superior to 90% for three reagents (Rota Strip (Cypress Diagnostics), 98.8%; Rotascreen (Microgen), 95.0%; VIKIA Rota/Adeno (bioMérieux), 92.5%); from 82.5% to 88.8% for three others (Diarlex with centrifugation (Orion Diagnostica), 88.8%; Combo Rota/Adeno (All Diag), 87.5%; Rota/Adeno Combi Stick (bmd), 82.5%) and only 70.0% for Diarlex with filtration vial (Orion Diagnostica). The evaluation of the specificity, performed on one hundred fresh rotavirus negative stools, did not show any false positives with any assay. Analysis of the different technical features of these tests showed that they are quick and suitable for a clinical laboratory and do not require expensive equipment.
• Emergence of Serotype G12 Rotaviruses, Hungary
K Bányai, Á Bogdán, P Kisfali, P Molnár, I Mihály, B Melegh,V Martella, JR. Gentsch and G Szücs
Emerg Infect Dis. 2007 Jun;13(6):916-9.
We describe the emergence of serotype G12 rotaviruses (67 [6.9%] of 971 specimens tested) among children hospitalized with rotavirus gastroenteritis in Hungary during 2005. These findings are consistent with recent reports of the possible global spread and increasing epidemiologic importance of these strains, which may have implications for current rotavirus vaccination strategies.
• Enterocolitis due to Simultaneous Infection with Rotavirus and Clostridium difficile in Adult and Pediatric Solid Organ Transplantation.
I Stelzmueller, S Wiesmayr, M Eller, M Fille, C Lass-Floerl & G Weiss, P Hengster, R Margreiter, H Bonatti
J Gastrointest Surg (2007) 11:911–917
Diarrhea is a well-known complication of immunosuppression but is also frequently caused by pathogens such as Clostridium difficile (CD) and rotavirus (RV). Three adult and five pediatric solid organ recipients (SORs) developed diarrhea with simultaneous identification of CD and RV. Rotavirus was identified using an immunochromatografic- or enzyme-linked
immunosorbent assay; CD was identified using a rapid immunoassay or enzyme immunoassay. One adult renal, one adult kidney–pancreas, one adult liver, and five pediatric liver recipients were affected. Onset of RV/CD infection ranged from 2 weeks to 4 years posttransplant. All patients presented with enterocolitis causing significant fluid and electrolyte loss. In adults, CD was treated with metronidazole and in children with oral vancomycin. RV infection was treated with fluid/electrolyte replacement. During diarrhea, a significant rise in tacrolimus serum level was noted. All patients cleared CD. One child developed recurrent episodes of RV infection and died from bacterial sepsis; the renal recipient died 6 months posttransplant from myocardial infarction. The remaining six patients are currently alive with well-functioning grafts. Simultaneous infection with CD and RV may lead to severe diarrhea in SORs. Both pathogens should be considered in SOR presenting with diarrhea.
• Rotavirus enteritis in solid organ transplant recipients: an underestimated problem?
Stelzmueller I, Wiesmayr S, Swenson BR, Biebl M, Goegele H, Margreiter R, Bonatti H.
Transpl Infect Dis.2007 Dec;9(4):281-5.
BACKGROUND: Diarrhea in solid organ transplantation can be a complication with a high morbidity and mortality. Rotavirus (RV) infection normally occurs in children up to 3 years of age and often presents with severe diarrhea; however, it can also affect adults. We investigated the prevalence and outcome of RV infections in both adult and pediatric patients after solid organ transplantation.
PATIENTS AND METHODS: Retrospective analysis of RV-related enteritis in solid organ transplant recipients with a minimum of a 1-year follow-up from a single center between 2000 and 2004.
RESULTS: Within our cohort of 1303 solid organ transplants, RV infection was observed in 19 patients (1.5%); 14 of these were liver recipients. Infection was most prevalent among pediatric liver recipients, with 52% (11/21) of the children affected. Five adults acquired the infection during their initial hospitalization. Two adult patients had to be readmitted following late-onset RV infection. In all cases, infection was self-limiting, but led to prolonged hospitalization because of significant loss of fluids and electrolytes.
CONCLUSIONS: RV enteritis is a common infection in pediatric solid organ recipients but may also affect adult patients.
• Rotavirus Gastroenteritis Leading to Secondary Bacteremia in Previously Healthy Infants
González-Carretero P, Noguera A, Fortuny C.
PEDIATRICS Vol. 118 No. 5 November 2006, pp. 2255a-2256
• Identification of risk factors associated with nosocomial infection by rotavirus P4G2, in a neonatal unit of a tertiary-care hospital.
Herruzo R, Omeñaca F, García S, Diez J, Sánchez-Fauquier A.
Clin Microbiol Infect. 2009 Mar;15(3):280-5.
A rotavirus outbreak in newborns admitted to the 'La Paz' University Hospital, Madrid was detected, followed up and controlled. Uninfected children were selected as control subjects. Samples of faeces were taken once or twice weekly from all the newborns, including those who were asymptomatic and who were admitted to the neonatal unit for early detection of rotavirus and the positive were separated from the rest of the neonates. Contact-related precautions were taken for all patients, and alcohol solutions were used for hand washing. During the months of the outbreak, 1773 children were admitted to the hospital, 131 of whom were affected by the rotavirus infection (7.4%). Of these, 72 (55%) had symptomatic infections. In the first month of the outbreak, nine cases of necrotizing enterocolitis were diagnosed (one patient developed massive intestinal necrosis). The infections (symptomatic and asymptomatic) presented a bimodal distribution caused by a new outbreak of rotavirus type P4G2 after two patients who had acquired the infection outside the hospital were admitted when the first outbreak was subsiding. The characteristics of cases and controls were analysed using bivariate and multivariate methods (non-conditional multivariate logistic regression) to identify four risk factors strongly associated with rotavirus infection: premature birth, infections other than rotavirus, malformation, and changes in glycaemia and/or presence of jaundice.
• Detecção de rotavírus por imunocromatografia: ensaio simples e de rápida execução / Rotavírus detection by imunocromatografic: simple assay with a fast execution
Gabbay Y, Morais M, Alves S, Oliveira K, Mascarenhas J, Linhares A.
Rev. para. Med 2005 19(1):13-18.
• Çocukluk Yaş Grubu Gastroenteritlerinde Rotavirüs Sıklığnın Araştırılması
Nazik H, İlktaç M, Öngen B.
ANKEM Derg. 2006;20(4):233–235