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Products  >  Human-Field  >  RESIST-4 O.K.N.V.  >  Science

 

RESIST-4 O.K.N.V. - Science

 

• Evaluation of the RESIST-4 O.K.N.V immunochromatographic lateral flow assay for the rapid detection of OXA-48, KPC, NDM and VIM carbapenemases from cultured isolates.

James Wesley MacDonald and Vindana Chibabhai.

Access Microbiology 2019;1

Abstract

Purpose This study aimed to evaluate the performance of the RESIST-4 O.K.N.V immunochromatographic lateral flow assay for the detection of OXA-48, KPC, NDM and VIM carbapenemases in 100 clinical Enterobacteriaceae isolates using solid culture media.
Methodology In total, 100 clinical Enterobacteriaceae isolates with characterized β-lactamase enzymes (OXA-48 n=46, KPC n =4, NDM n =43 and VIM n =10) were evaluated using the RESIST-4 O.K.N.V assay. The assay was also evaluated using carbapenem-sensitive control strains and confirmed non-carbapenemase-producing Enterobacteriaceae clinical isolates resistant to carbapenems. Inter-rater agreement of the test was evaluated by four different users who tested 11 randomly selected isolates daily over 3 days.
Results Overall accuracy of the assay was 99.5  %. For the detection of KPC, OXA-48 and its variants and VIM the assay correctly identified 100  % of the isolates when compared to PCR. Initial performance for NDM detection was sensitivity=95.3 %, specificity=100  %. Two PCR positive Providencia rettgeri isolates rendered false negative results on the assay. Retesting from a carbapenem zone of inhibition rendered a positive result for both isolates increasing the sensitivity to 100  %. No false positive results or cross reactions were detected.
Conclusion The RESIST-4 O.K.N.V is reliable, sensitive and specific for the detection of OXA-48, KPC, NDM and VIM carbapenemases. Further evaluation on improving NDM detection in organisms from the Proteeae tribe is warranted to determine optimal test conditions.

https://www.microbiologyresearch.org/content/journal/acmi/10.1099/acmi.0.000031?crawler=redirect&mimetype=application/pdf

 

• Detection of carbapenemase production in pseudomonas aeruginosa in a tertiary care centre.

T. Pilate, S. Desmet.

Annual Meeting of the Royal Belgian Society of Laboratory Medicine November 15th, 2019 Belgium.

 Poster

 

• Unravelling ceftazidime/avibactam resistance of KPC-28, a KPC-2 variant lacking carbapenemase activity.

Oueslati S, Iorga BI, Tlili L, Exilie C, Zavala A, Dortet L, Jousset AB, Bernabeu S, Bonnin RA, Naas T.

J Antimicrob Chemother. 2019 Aug 1;74(8):2239-2246

Abstract

BACKGROUND:KPC-like carbapenemases have spread worldwide with more than 30 variants identified that differ by single or double amino-acid substitutions.
OBJECTIVES:To describe the steady-state kinetic parameters of KPC-28, which differs from KPC-2 by a H274Y substitution and the deletion of two amino acids (Δ242-GT-243).
METHODS:The blaKPC-2, blaKPC-3, blaKPC-14 and blaKPC-28 genes were cloned into a pTOPO vector for susceptibility testing or into pET41b for overexpression, purification and subsequent kinetic parameter (Km, kcat) determination. Molecular docking experiments were performed to explore the role of the amino-acid changes in the carbapenemase activity.
RESULTS:Susceptibility testing revealed that Escherichia coli producing KPC-28 displayed MICs that were lower for carbapenems and higher for ceftazidime and ceftazidime/avibactam as compared with KPC-2. The catalytic efficiencies of KPC-28 and KPC-14 for imipenem were 700-fold and 200-fold lower, respectively, than those of KPC-2, suggesting that Δ242-GT-243 in KPC-28 and KPC-14 is responsible for reduced carbapenem hydrolysis. Similarly, the H274Y substitution resulted in KPC-28 in a 50-fold increase in ceftazidime hydrolysis that was strongly reversed by clavulanate.
CONCLUSIONS:We have shown that KPC-28 lacks carbapenemase activity, has increased ceftazidime hydrolytic activity and is strongly inhibited by clavulanate. KPC-28-producing E. coli isolates display an avibactam-resistant ESBL profile, which may be wrongly identified by molecular and immunochromatographic assays as the presence of a carbapenemase. Accordingly, confirmation of carbapenem hydrolysis will be mandatory with assays based solely on blaKPC gene or gene product detection.

https://www.ncbi.nlm.nih.gov/pubmed/31127297

 

• Detection of Carbapenemase-Producing Enterobacteriaceae in Positive Blood Culture Using an Immunochromatographic RESIST-4 O.K.N.V. Assay.

Cointe A, Bonacorsi S, Truong J, Hobson C, Doit C, Monjault A, Bidet P, Birgy A.

Antimicrob Agents Chemother. 2018 Nov 26;62(12). pii: e01828-18

Abstract

We evaluated the performance of the RESIST-4 O.K.N.V. assay (Coris) with 98 isolates to detect OXA-48-like and KPC-, NDM-, and VIM-type carbapenemases directly on positive human blood cultures. OXA-48-like and KPC-type isolates were correctly detected, but the detection of NDM- and VIM-type carbapenemases was weak and variable. We show that repeating the test on a 4-h subculture improves the detection of NDM- and VIM-type carbapenemases to 100%.

https://www.ncbi.nlm.nih.gov/pubmed/30249695

 

• Comparison of five methods for detection of carbapenemases in Enterobacterales with proposal of a new algorithm.

Baeza LL, Pfennigwerth N, Greissl C, Göttig S, Saleh A, Stelzer Y, Gatermann SG, Hamprecht A.

Clin Microbiol Infect. 2019 Mar 18. pii: S1198-743X(19)30104-1.

Abstract

OBJECTIVES: The aim of this study was to evaluate the performance of five different carbapenemase tests and to develop an algorithm which will permit the detection of most common and rare carbapenemases in routine microbiology laboratories.

MATERIALS AND METHODS: The immunochromatographic tests CARBA-5 (NG), RESIST-4 O.K.N.V. (Coris), the colorimetric β-CARBA (BioRad), a newly developed carbapenem-inactivation method (CIM) supplemented with zinc (zCIM) and the Xpert Carba-R (Cepheid) were challenged with a collection of 189 molecularly characterized Enterobacterales isolates, including 146 carbapenemase producers (CPE): VIM (n=48), OXA-48-like (n=40), NDM (n=29), KPC (n=13), IMI (n=9), IMP (n=9), OXA-58 (n=2) and GES (n=2).

RESULTS: The overall sensitivity/specificity values for the five carbapenemase detection tests were 84.2%(CI 77.6%-89.2%)/100%(CI 91.8%-100%) for RESIST-4, 88.2%(CI 82.1%-92.4%)/100%(CI 91.8%-100%) for CARBA-5, 88.2%(CI 82.1%-92.4%)/100%(CI 91.8%-100%) for Xpert Carba-R, 73.7%(CI 66.2%-80.0%)/100%(CI 93.4%-99.0%) for β-CARBA and 97.4%(CI 87.9%-99.6%)/97.7%(CI 87.9%-99.6%) for zCIM. The four common carbapenemases (KPC, OXA-48-like, NDM and VIM) were detected with ≥97.6% sensitivity by all tests except for β-CARBA (76.6%[CI 68.4%-83.2%]). IMI and GES were only detected by zCIM (sensitivity 90.9%[CI 62.3%-98.4%]). Based on these results a new algorithm was developed, consisting of an immunochromatographic assay as the first test followed by zCIM, which allows detection of 99.3% of all carbapenemases assessed.

CONCLUSIONS: Except for β-CARBA, all methods showed excellent sensitivity/specificity for the detection of the four most frequent carbapenemases. With the new algorithm, rare variants can also be detected. It is rapid, simple and inexpensive and can be performed in any microbiology laboratory, as no PCR equipment is required.

https://www.ncbi.nlm.nih.gov/pubmed/30898725

 

 

• Evaluation of a novel immunochromatographic lateral flow assay for rapid detection of OXA-48, NDM, KPC and VIM carbapenemases in multidrug-resistant Enterobacteriaceae.

Rösner S, Kamalanabhaiah S, Küsters U, Kolbert M, Pfennigwerth N, Mack D.

J Med Microbiol. 2019 Mar;68(3):379-381.

Abstract

PURPOSE:The global spread and increasing clinical impact of carbapenemase-producing bacteria are alarming. Rapid diagnostic techniques for carbapenemase detection are of the utmost importance to prevent delays in efficient antibiotic therapy and the control of spread in hospitals. Recently, multiplex immunochromatographic lateral flow tests (ICTs) for the fast detection of carbapenemase-producers have become commercially available. We evaluated a novel multiplex ICT for the rapid detection of OXA-48, KPC, NDM and VIM carbapenemases.

METHODOLOGY:One hundred well-characterized multidrug-resistant Enterobacteriaceae were analysed by RESIST-4 (Coris, BioConcept, Gembloux, Belgium). The reference standard included confirmation at the molecular level at the German National Reference Laboratory for multidrug-resistant Gram-negative bacteria (Ruhr-University Bochum, Germany).Results/Key findings. The tested ICT was highly reliable, showing 100 % sensitivity and 100 % specificity.

CONCLUSION:As it is fast, easy to perform and has few technical requirements, the ICT represents a good opportunity to improve turnaround times and patient care for the clinical microbiology laboratory.

https://www.ncbi.nlm.nih.gov/pubmed/30663953

 

 

• Evaluation of the RESIST-4 K-SeT assay, a multiplex immunochromatographic assay for the rapid detection of OXA-48-like, KPC, VIM and NDM carbapenemases.

Glupczynski Y, Evrard S, Huang TD, Bogaerts P.

J Antimicrob Chemother. 2019 Feb 6. doi: 10.1093.

Abstract

OBJECTIVES: Accurate and fast identification of carbapenemase producers is essential for optimal patient management. Here, a new lateral flow immunochromatographic RESIST-4 K-SeT assay was assessed for the detection of carbapenemases in Enterobacteriaceae and non-fermenters.

METHODS: The RESIST-4 K-SeT assay targets OXA-48-like, KPC, VIM and NDM, but not IMP carbapenemases. The assay was first evaluated using a collection of isolates with well-characterized resistance mechanisms to β-lactams (n = 134) and against an international external quality assessment carbapenemase panel (n = 8). The assay was then challenged prospectively using 345 consecutive, non-duplicate isolates including 279 Enterobacteriaceae and 66 non-fermenters (mostly Pseudomonas spp.) that were sent to the Belgian National Reference Centre for identification of the mechanisms related to carbapenem resistance.

RESULTS: Globally, for the collection of retrospective and prospective clinical isolates (n = 479), the assay showed a sensitivity ranging from 99% for the detection of VIM to 100% for the detection of OXA-48-like, KPC and NDM carbapenemase-producing strains. The specificity was 100% for each carbapenemase and a perfect match in results was observed for the external quality assessment for the carbapenemases targeted by the assay.

CONCLUSIONS:The RESIST-4 K-SeT assay is a valuable alternative for detection and identification of carbapenemases from culture isolates compared with the more costly molecular assays, which may also further require skilled staff and dedicated facilities.

https://www.ncbi.nlm.nih.gov/pubmed/30753488

 

 

• Détection directe des carbapénèmases sur hémoculture par test immunochromatographique RESIST-4-O.K.N.V.

Cointe A, Bonacorsi S, Truong J, Hobson C, Doit C, Monjault A, Bidet P, Birgy A.

38e Réunion interdisciplinaire de chimiothérapie anti-infectieuse 17-18 décembre, 2018 Paris.

 Poster

 

 

• Évaluation de deux kits immuno-chromatographiques pour la détection des souches d’Entérobactéries Productrices de Carbapénèmase.

Miltgen G., Smith A., Hibon V., MangataE., Bencimon C., Ramiandrisoa M., Picot S., Lignereux A., Masson G, Leclaire A, Seraphin P, Traversier N, Roquebert B, Jaffar-Bandjee MC, Belmonte O.

38e Réunion interdisciplinaire de chimiothérapie anti-infectieuse 17-18 décembre, 2018 Paris.

 Poster

 

 

• Evaluation des tests immunochromatographiquesRESIST-4 O.K.N.V. et NG-Test CARBA-5
pour la détection des entérobactéries productrices de carbapénémases.

AUBRY C., LAVIGNE JP., PANTEL A.

38e Réunion interdisciplinaire de chimiothérapie anti-infectieuse 17-18 décembre, 2018 Paris.

 Poster

 

 

• VALUTAZIONE DEL KIT RESIST-4 O.K.N.V. (CORIS BIOCONCEPT) PER LA RILEVAZIONE DELLE CARBAPENEMASI NEI BATTERI GRAM-NEGATIVI PRODUTTORI DI CARBAPENEMASI.

KOLENDA C., BENOIT R., CARRICAJO A., BONNET R., DAUWALDER O., LAURENT F.

38e Réunion interdisciplinaire de chimiothérapie anti-infectieuse 17-18 décembre, 2018 Paris.

 Poster

 

• Rapid detection of OXA-48-like, KPC, NDM, and VIM carbapenemases in Enterobacterales by a new multiplex immunochromatographic test.

Greissl C,  Saleh A and Hamprecht A.

Eur J Clin Microbiol Infect Dis. 2018 Nov 17. doi: 10.1007/s10096-018-3432-2.

Abstract
The rapid detection of carbapenemase-producing Gram-negative bacteria is indispensable to optimize treatment and avoid the further spread of these organisms. While phenotypic tests are time-consuming and PCR is expensive and not available in many routine laboratories, immunochromatographic tests (ICT) can provide rapid results at moderate cost. The aim of this study was to determine the performance of the new ICT RESIST-4 O.K.N.V. K-SeT (Coris BioConcept, Gembloux, Belgium) which can detect the four most prevalent carbapenemases: OXA-48-like, KPC, NDM, and VIM. Additionally, we analyzed the impact of different culture conditions on the sensitivity. The new ICT was challenged with 169 carbapenem-resistant isolates. Of these, 125 were carbapenemase producers: 43 OXA-48-like, 15 KPC, 29 NDM, and 43 VIM. The ICT correctly detected 129 of the 130 carbapenemases resulting in a sensitivity of 99.2% and specificity of 100% when tested from Mueller-Hinton agar (MHA). The sensitivity of the assay increased to 100% when performed from zinc-supplemented MHA and sheep blood agar (SBA) or when the inoculum was harvested from the inhibition zone of an ertapenem disk. All carbapenemase-negative carbapenem-resistant bacteria tested negative and no cross-reaction was observed. The new ICT is an excellent test for rapid diagnostic of carbapenemase-producing Gram-negatives in the routine laboratory. It is easy to handle and provides rapid results with a high sensitivity. For best results, we recommend to obtain the inoculum from a medium with sufficient zinc or from the inhibition zone of an ertapenem disk.

https://www.ncbi.nlm.nih.gov/pubmed/30448931

 

 

• Evaluation of the New Multiplex Immunochromatographic O.K.N.V. K-SeT Assay for Rapid Detection of OXA-48-like, KPC, NDM, and VIM Carbapenemases.

Kolenda C, Benoit R, Carricajo A, Bonnet R, Dauwalder O, Laurent F.

J Clin Microbiol. 2018 Oct 25;56(11). pii: e01247-18.

Abstract
No available

https://www.ncbi.nlm.nih.gov/pubmed/30185510

 

 

• VALUTAZIONE DEL KIT RESIST-4 O.K.N.V. (CORIS BIOCONCEPT) PER LA RILEVAZIONE DELLE CARBAPENEMASI NEI BATTERI GRAM-NEGATIVI PRODUTTORI DI CARBAPENEMASI.

E. Meroni, V. Viaggi, N. Corbo, L. Principe, B. Pini and F. Luzzaro.

3XLVII Congresso Nazionale AMCLI, 10-13 Novembre 2018, Rimini, Italia.

 Poster

 

 

• Detection of carbapenemases-producing Enterobacteriaceae in positive blood culture using Immunochromatographic RESIST-4 O.K.N.V assay.

Cointe A, Bonacorsi S, Truong J, Hobson C, Doit C, Monjault A, Bidet P, Birgy A.

Antimicrob Agents Chemother. 2018 Sep 24. pii: AAC.01828-18. doi: 10.1128/AAC.01828-18.

Abstract
We evaluated the performances of the RESIST-4.O.K.N.V assay (Coris®) on 98 isolates to detect OXA-48-like, KPC-, NDM- and VIM-type carbapenemases directly on positive human blood cultures. OXA-48-like and KPC-type isolates were correctly detected but detection of NDM and VIM-type was weak and variable. We show that repeating the test on a 4-hour subculture improves the detection of NDM- and VIM-type to 100%.

https://www.ncbi.nlm.nih.gov/pubmed/30249695
 

 

• P2333 - A new method for detection of carbapenemase-producing Enterobacteriaceae carriage.

K.Villageois-Tran, A.A. Mariaggi, A. Godmer, M. Lambot, T. Leclitpeux, G. Arlet, Y. Benzerara and S. Gallah.

28th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 21 – 24, 2018.

 


• P2328 – Evaluation of the NV K-SeT, a new lateral flow assay for the detection of VIM and NDM producing bacteria.

Y. Glupczynski, T.D. Huang, S. Evrard and P. Bogaerts.

28th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 21 – 24, 2018.

Identifying the ‘Big Four’ carbapenemases in the routine laboratory
Pathology in Practice 2017 Aug.: 18 (3) 46–7.
https://www.pathologyinpractice.com/features


• Evaluation of a new lateral flow assay for the detection of VIM-producing bacteria.

P. Bogaerts, S. Evrard, L. Denorme, Q. Gilleman, P. Mertens, T.-D. Huang,1 and Y. Glupczynski.

37e Réunion interdisciplinaire de chimiothérapie anti-infectieuse 18-19 décembre, 2017 Paris..

 Poster

 

• Confronto tra tecniche per il rilevamento di Enterobacteriaceae produttrici di carbapenemasi.

Brossa S., Cipriani R., Zanotto E., Quaranta M.R., Morcinelli M., Zaccaria T., Cavallo R.  - Poster P084.

XLVI Congresso Nazionale dell'Associazione Microbiologi Clinici Italiani. Rimini, 11-14 Nov, 2017.

 Poster

 

• Valutazione del kit RESIST-3 O.K.N. K-SeT (Coris Bioconcept – Biolife) per la rilevazione delle carbapenemasi negli enterobatteri  - Poster P070.

Elisa Meroni, Carola Mauri, Valentina Viaggi, Beatrice Pini, Luigi Principe, Francesco Luzzaro.

XLVI Congresso Nazionale dell'Associazione Microbiologi Clinici Italiani. Rimini, 11-14 Nov, 2017.

 Poster

 

• Sorveglianza attiva degli Enterobatteri produttori di carbapenemasi in terapia intensiva.

De Rosa P., Abagnale I., Fusco A., Spanò A., Filosa A. – Poster007.

XLVI Congresso Nazionale dell'Associazione Microbiologi Clinici Italiani. Rimini, 11-14 Nov, 2017.

 Poster

 

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