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Products  >  Human-Field  >  KPC  >  Science

KPC - Science

 

• Evaluation of a rapid immunochromatographic test for detection of KPC in clinical isolates of Enterobacteriaceae and Pseudomonas species.

Wozniak A, Paillavil B, Legarraga P, Zumarán C, Prado S, García P.

Diagn Microbiol Infect Dis. 2019 Oct;95(2):131-133. doi: 10.1016/j.diagmicrobio.2019.05.009. Epub 2019 May 24.

Abstract
The KPC K-SeT immunochromatographic test (Coris BioConcept®, Gembloux, Belgium) has been widely used for detection of KPC in Enterobacteriaceae with reported sensitivities and specificities of 100%. However, to our knowledge, there are no reports of its use in KPC-positive Pseudomonas species. We evaluated the KPC K-SeT test in 36 clinical isolates of Enterobacteriaceae (21 KPC-positive and 15 KPC-negative) and 20 Pseudomonas species (5 KPC-positive and 15 KPC-negative) using conventional PCR for carbapenemase genes as the reference method. The KPC K-SeT test detected 25 out of 26 KPC-positive isolates (96.1%). The undetected isolate was 1 P. aeruginosa bearing the mutation D179Y in the omega loop region of KPC-2 carbapenemase. This mutation was already reported in Enterobacteriaceae as conferring resistance to ceftazidime-avibactam. To our knowledge, this is the first report of evaluation of KPC K-SeT test in KPC-positive P. aeruginosa isolates.

https://www.ncbi.nlm.nih.gov/pubmed/31208819

 

• KPC-31 expressed in a ceftazidime/avibactam-resistant Klebsiella pneumoniae is associated with relevant detection issues.

Antonelli A, Giani T, Di Pilato V, Riccobono E, Perriello G, Mencacci A, Rossolini GM.

J Antimicrob Chemother. 2019 Aug 1;74(8):2464-2466.

No abstract.

https://www.ncbi.nlm.nih.gov/pubmed/31318973

 

• Current fast methods for the identification of carbapenemase enzymes in clinical laboratory.

D'Aleo F., Ceccarelli M., Facciolà A., Venanzi Rullo E., Paolucci I., Lo Presti Costantino M. R., Fazii P., Conte M., Pellicanò G. F.

Infectious Diseases & Tropical Medicine 2018; 4 (2): e470.

Abstract
Introduction: Multidrug  resistance  is  growing  at  an  alarming  rate  among  a  variety  of  bacterial  species,  causing  infections,  which  threaten  the  lives  of  patients.  Detection  of  metal-β-lactamase  producers  (IMP,  VIM, NDM) and of KPC producers may be based on the inhibitory properties of several molecules but re-quires additional expertise and time. Several authors describe the use of new rapid identification methods. The aim of our review is to revise the new rapid methods for the detection of carbapenemases and their identification by Gram-negative bacilli.

https://www.infectiousjournal.com/wp-content/uploads/sites/6/2018/06/e470-Current-fast-methods-for-the-identification-of-carbapenemase-enzymes-in-clinical-laboratory.pdf

 

• Evaluation of the KPC K-SeT® immunochromatographic assay for the rapid detection of KPC carbapenemase producers from positive blood cultures.

Riccobono E, Antonelli A, Pecile P, Bogaerts P, D'Andrea MM, Rossolini GM.

J Antimicrob Chemother. 2018 Feb 1;73(2):539-540. doi: 10.1093/jac/dkx406.

In this work, we evaluated the performance of the KPC K-SeT® assay for the detection of KPC-type carbapenemases directly from positive blood cultures.

https://www.ncbi.nlm.nih.gov/pubmed/29126269

 

• Evaluation of a rapid immunochromatographic test for detection of distinct variants of Klebsiella pneumoniae carbapenemase (KPC) in Enterobacteriaceae.

Ramos AC, Gales AC, Monteiro J, Silbert S, Chagas-Neto T, Machado AMO, Carvalhaes CG.

J Microbiol Methods. 2017 Nov;142:1-3.

Abstract
The rapid detection of KPC-producing Enterobacteriaceae by microbiology laboratories has been required for infectious control programs. Herein we evaluated the performance of a novel immunochromatographic test for detecting KPC-2-, KPC-3-, KPC-4-, KPC-6-, KPC-7-, KPC-8-, and KPC-11-producing isolates and the influence of different growth media on the test performance.

http://www.sciencedirect.com/science/article/pii/S0167701217302245?via%3Dihub

 

• Evaluation of Letitest KPC K-SeT rapid immunochromatographic test for detection of KPC carbapenemase.

M. Pinto da Costa, G. Abreu and A. Lira.

9a Reuniao cientifica da sociedade portuguesa de medicina laboratorial  Abril 7-8, 2017.

 Poster

 

• Comparison of the Novel Oxa-48 and Kpc K-SeT Assay, and Blue-Carba Test for the Detection of Carbapenemase-Producing Enterobacteriaceae Using PCR as a Reference Method.

Erdem F, Abulaila A, Aktas Z, Oncul O.

Clin Lab. 2017 Mar 1;63(3):515-522.

Abstract

BACKGROUND:

Rapid, simple, and accurate laboratory detection of carbapenemases is very important for proper antibiotic therapy and infection control.

METHODS:

In this study, carbapenem-nonsusceptible Enterobacteriaceae (CRE) isolates were used to evaluate the performance of a new lateral flow immunochromatographic (IC) assay, the OXA-48 and KPC K-SeT assay, and modified Blue-Carba test (BCT) for the rapid detection of OXA-48 carbapenemase in comparison with polymerase chain reaction (PCR) amplification. These CREs of various enterobacterial species were isolated from various clinical samples including OXA-48 (47), NDM-1 (6), KPC-1 (1), IMP-1 (1), VIM-2,-4 (2), IMP-2 (1), OXA-51 (1), and OXA-23 (1) producers.

RESULTS:

The OXA-48 K-SeT test detected all OXA-48 carbapenemase producers with 100% sensitivity and specificity. The BCT detected carbapenemase producers with 93% sensitivity and 100% specificity.

CONCLUSIONS:

Both IC assays and BCT tests have good performance and are easy to perform, rapid, simple to interpret, and highly sensitive. We suggest that BCT can be used initially as an accurate, inexpensive, and rapid phenotypic confirmation test to identify Class A, B, and D

https://www.ncbi.nlm.nih.gov/pubmed/28271693

 

• Evaluation of Coris BioConcept’s KPC K-SeT Assay for Rapid Accurate Distinction of KPC Carbapenemase-Producing Organisms (CPO) from other CPO Genotypes and from Non-CPO

B. M. Willey, S. Lee, V. Koren, B. Gascon, S. Surangiwala, J. Bartoszko, H. C. Kim, P. Lo, S. M. Poutanen

27th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 22 – 25, 2017

 Poster

 

• Rapid identification of KPC carbapenemase on extensively drug-resistant bacterial colony in one Regional Teaching Hospital of Northern Taiwan

J.Y. Chen

The 8th Asia-Pacific Forum of Medical Laboratory Science Apr. 16-17, 2016, Taichung Taiwan

 Poster

 

• Evaluation of the K-SeT R.E.S.I.S.T. immunochromatographic assay for the rapid detecion ok KPC and OXA-48-like carbapenemase.

Danièle Meunier, Anna Vickers, Rachel Pike, Robert L.Hill, Neil Woodford, Katie L. Hopkins.

J Antimicrob Chemother. 2016 April 26 doi:10.1093/jac/dkw113

J. Antimicrob. Chemother.-2016-Meunier-jac-dkw113

 

• P1022 – Impact of the isolation medium for the detection of OXA-48 and KPC-producing Gram negative bacteria by immunochromatographic assays

P. Bogaerts, S. Evrard, M. Dozen, TD. Huang and Y. Glupczynski

26th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 09 – 12, 2016

 Poster P1022

 

• Evaluation of two novel commercial immunochromatographic assays for the direct detection of OXA-48 and of KPC carbapenemases from pure culture

Y. Glupczynski, S Evrard,  I Otte,  TD  Huang, P Mertens, T Leclipteux, P Bogaerts

35e Réunion interdisciplinaire de chimiothérapie anti-infectieuse 13 - 15 décembre, 2015 Paris.

 Poster403

 

• Développement de nouveaux tests immunochromatographiques pour l’identification rapide des carbapénèmases OXA-48 et KPC produites par les Entérobactéries.

I. Ote, P. Bogaerts, C. Borlon, L. Denorme, C. Thunissen, S. Evrard, Y. Glupczynski, P. Mertens.

35e Réunion interdisciplinaire de chimiothérapie anti-infectieuse 13 - 15 décembre, 2015 Paris.

 Poster386

 

• Evaluation of two new commercial immunochromatographic assays for the rapid detection of OXA-48 and KPC carbapenemases from cultured bacteria. 

Glupczynski Y, Evrard S, Ote I, Mertens P, Huang TD, Leclipteux T, Bogaerts P.

J Antimicrob Chemother. 2016 Jan 28. pii: dkv472. [Epub ahead of print]

https://www.ncbi.nlm.nih.gov/pubmed/26825120

Abstract :
BACKGROUND:
Rapid detection and confirmation of carbapenemases remains very challenging for diagnostic laboratories.
OBJECTIVES:
The objective of this study was to assess the performance of two new immunochromatographic (IC) commercial assays for the rapid detection of OXA-48-producing and KPC-producing Enterobacteriaceae in pure bacterial isolates.
METHODS:
A panel of 92 bacterial isolates predominantly including carbapenem-non-susceptible Enterobacteriaceae with previously defined carbapenem resistance mechanisms was tested. Then, 342 consecutive carbapenem-non-susceptible Enterobacteriaceae isolates referred to the reference laboratory were investigated prospectively in parallel with other phenotypic tests and with multiplex PCR and sequencing as the gold standard.
RESULTS:
In the collection panel, each of the two IC assays correctly detected all 30 OXA-48-like-producing isolates and 25 KPC-producing isolates, whatever the species, their association with other β-lactamases and the level of resistance to carbapenems. All other carbapenemase producers and all non-carbapenemase-producing isolates yielded negative results with both tests. In the prospective evaluation, all OXA-48-like-producing Enterobacteriaceae isolates (n = 130) and KPC-producing Enterobacteriaceae isolates (n = 33) were correctly detected by the individual IC assays, while 179 non-OXA-48-like-producing and non-KPC-producing strains (137 non-carbapenemase producers and 42 isolates belonging to other carbapenemase family types) yielded negative results. Thus, each assay yielded 100% sensitivity and 100% specificity for the detection of OXA-48-like or KPC enzymes, respectively, at 15 min.
CONCLUSIONS:
The two IC assays allow rapid and reliable direct confirmation of OXA-48 and KPC carbapenemases from culture colonies and appear to be very useful additions to the existing tests, obviating the need for more costly characterization by molecular amplification methods.

 

• La rilevazione delle resistenze ai carbapenemi negli enterobatteri con il test immunocromatograpico KPC K-SeT (Coris, BioConcept). 

Cosentino M, Corbellini S, Passera M, Vailati F, Farina C

XLIV Congresso Nazionale AMCLI  18-21 Ottobre, 2015  Rimini - PALACONGRESSI

 Palacongressi 2015

 

• Development of a novel immunochromatographic combo test for rapid identification of OXA-­48 and KPC carbapenemases in Enterobacteriaceae. 

Borlon C, Denorme L, Evrard S, Ote I, Bogaerts P, Glupczynski Y, Mertens P

115th American Society for Microbiology May 30 - June 2, 2015 New Orleans

 ASM-2015

 

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