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Products  >  Human-Field  >  RESIST-5 O.O.K.N.V.  >  Science

 

RESIST-5 O.O.K.N.V. - Science

 

• Détection directe des carbapénèmases sur hémoculture par test immunochromatographique RESIST-4-O.K.N.V.

Cointe A, Bonacorsi S, Truong J, Hobson C, Doit C, Monjault A, Bidet P, Birgy A.

38e Réunion interdisciplinaire de chimiothérapie anti-infectieuse 17-18 décembre, 2018 Paris.

 Poster

 

 

• Évaluation de deux kits immuno-chromatographiques pour la détection des souches d’Entérobactéries Productrices de Carbapénèmase.

Miltgen G., Smith A., Hibon V., MangataE., Bencimon C., Ramiandrisoa M., Picot S., Lignereux A., Masson G, Leclaire A, Seraphin P, Traversier N, Roquebert B, Jaffar-Bandjee MC, Belmonte O.

38e Réunion interdisciplinaire de chimiothérapie anti-infectieuse 17-18 décembre, 2018 Paris.

 Poster

 

 

• Evaluation des tests immunochromatographiquesRESIST-4 O.K.N.V. et NG-Test CARBA-5
pour la détection des entérobactéries productrices de carbapénémases.

AUBRY C., LAVIGNE JP., PANTEL A.

38e Réunion interdisciplinaire de chimiothérapie anti-infectieuse 17-18 décembre, 2018 Paris.

 Poster

 

 

• VALUTAZIONE DEL KIT RESIST-4 O.K.N.V. (CORIS BIOCONCEPT) PER LA RILEVAZIONE DELLE CARBAPENEMASI NEI BATTERI GRAM-NEGATIVI PRODUTTORI DI CARBAPENEMASI.

KOLENDA C., BENOIT R., CARRICAJO A., BONNET R., DAUWALDER O., LAURENT F.

38e Réunion interdisciplinaire de chimiothérapie anti-infectieuse 17-18 décembre, 2018 Paris.

 Poster

 

 

• P2332 – Carbapenemase-producing Enterobacteriaceae (CPE) detection directly from surveillance rectal swab bu immunochromatographic test.

C.R.L. Fonseca, A.V. Braga, C.R. Ferrari, T. Chagas-Neto, A.C. Ramos, A. C.Gales, A. Andriolo and C.G. Carvalhaes.

28th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 21 – 24, 2018

 Poster
 


• P0293 – Reduction in the detection time of OXA-48, OXA-163 and KPC carbapenemases performed with a novel immunochromatographic lateral flow assay from 2-hour-old cultures (early cultures).

F. Pasteran, L. Denorme, Q. Gilleman, S. Gomez, E. Albornoz, Y. Glupczynski, P. Bogaerts, P. Mertens and A. Corso.

27th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 22 – 25, 2017

 Poster
 

 

• Rapid Identification of OXA-48 and OXA-163 Subfamilies in Carbapenem-Resistant Gram-Negative Bacilli with a Novel Immunochromatographic Lateral Flow Assay.

Pasteran F, Denorme L, Ote I, Gomez S, De Belder D, Glupczynski Y, Bogaerts P, Ghiglione B, Power P, Mertens P, Corso A.

J Clin Microbiol. 2016 Nov;54(11):2832-2836. Epub 2016 Aug 17.

Abstract
TWe assessed a novel immunochromatographic lateral flow assay for direct identification of OXA-48-like carbapenemases and accurate differentiation of allele variants with distinct substrate profiles (OXA-48 or OXA-163 subfamilies). The assay allowed rapid (less than 4 min) and reliable direct confirmation of OXA-163- and/or OXA-48-like enzymes (with 100% sensitivity and 100% specificity) from cultured colonies that were recovered from both solid medium and spiked blood culture bottles.

https://www.ncbi.nlm.nih.gov/pubmed/27535687


 

• Rapid Identification of OXA-48-like, OXA-163 subfamily and KPC carbapenemases in Enterobacteriaceae by immunochromatography.

L. Denorme, I. Ote, F. Pasteran, S. Gomez, A. Corso, P. Mertens.

 Poster

 

 

• Rapid detection of OXA-48-like, KPC, NDM, and VIM carbapenemases in Enterobacterales by a new multiplex immunochromatographic test.

Greissl C,  Saleh A and Hamprecht A.

Eur J Clin Microbiol Infect Dis. 2018 Nov 17. doi: 10.1007/s10096-018-3432-2.

Abstract
The rapid detection of carbapenemase-producing Gram-negative bacteria is indispensable to optimize treatment and avoid the further spread of these organisms. While phenotypic tests are time-consuming and PCR is expensive and not available in many routine laboratories, immunochromatographic tests (ICT) can provide rapid results at moderate cost. The aim of this study was to determine the performance of the new ICT RESIST-4 O.K.N.V. K-SeT (Coris BioConcept, Gembloux, Belgium) which can detect the four most prevalent carbapenemases: OXA-48-like, KPC, NDM, and VIM. Additionally, we analyzed the impact of different culture conditions on the sensitivity. The new ICT was challenged with 169 carbapenem-resistant isolates. Of these, 125 were carbapenemase producers: 43 OXA-48-like, 15 KPC, 29 NDM, and 43 VIM. The ICT correctly detected 129 of the 130 carbapenemases resulting in a sensitivity of 99.2% and specificity of 100% when tested from Mueller-Hinton agar (MHA). The sensitivity of the assay increased to 100% when performed from zinc-supplemented MHA and sheep blood agar (SBA) or when the inoculum was harvested from the inhibition zone of an ertapenem disk. All carbapenemase-negative carbapenem-resistant bacteria tested negative and no cross-reaction was observed. The new ICT is an excellent test for rapid diagnostic of carbapenemase-producing Gram-negatives in the routine laboratory. It is easy to handle and provides rapid results with a high sensitivity. For best results, we recommend to obtain the inoculum from a medium with sufficient zinc or from the inhibition zone of an ertapenem disk.

https://www.ncbi.nlm.nih.gov/pubmed/30448931

 

 

• Evaluation of the New Multiplex Immunochromatographic O.K.N.V. K-SeT Assay for Rapid Detection of OXA-48-like, KPC, NDM, and VIM Carbapenemases.

Kolenda C, Benoit R, Carricajo A, Bonnet R, Dauwalder O, Laurent F.

J Clin Microbiol. 2018 Oct 25;56(11). pii: e01247-18.

Abstract
No available

https://www.ncbi.nlm.nih.gov/pubmed/30185510

 

 

• VALUTAZIONE DEL KIT RESIST-4 O.K.N.V. (CORIS BIOCONCEPT) PER LA RILEVAZIONE DELLE CARBAPENEMASI NEI BATTERI GRAM-NEGATIVI PRODUTTORI DI CARBAPENEMASI.

E. Meroni, V. Viaggi, N. Corbo, L. Principe, B. Pini and F. Luzzaro.

3XLVII Congresso Nazionale AMCLI, 10-13 Novembre 2018, Rimini, Italia.

 Poster

 

 

• Detection of carbapenemases-producing Enterobacteriaceae in positive blood culture using Immunochromatographic RESIST-4 O.K.N.V assay.

Cointe A, Bonacorsi S, Truong J, Hobson C, Doit C, Monjault A, Bidet P, Birgy A.

Antimicrob Agents Chemother. 2018 Sep 24. pii: AAC.01828-18. doi: 10.1128/AAC.01828-18.

Abstract
We evaluated the performances of the RESIST-4.O.K.N.V assay (Coris®) on 98 isolates to detect OXA-48-like, KPC-, NDM- and VIM-type carbapenemases directly on positive human blood cultures. OXA-48-like and KPC-type isolates were correctly detected but detection of NDM and VIM-type was weak and variable. We show that repeating the test on a 4-hour subculture improves the detection of NDM- and VIM-type to 100%.

https://www.ncbi.nlm.nih.gov/pubmed/30249695
 

 

• P2333 - A new method for detection of carbapenemase-producing Enterobacteriaceae carriage.

K.Villageois-Tran, A.A. Mariaggi, A. Godmer, M. Lambot, T. Leclitpeux, G. Arlet, Y. Benzerara and S. Gallah.

28th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 21 – 24, 2018.

 


• P2328 – Evaluation of the NV K-SeT, a new lateral flow assay for the detection of VIM and NDM producing bacteria.

Y. Glupczynski, T.D. Huang, S. Evrard and P. Bogaerts.

28th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 21 – 24, 2018.

Identifying the ‘Big Four’ carbapenemases in the routine laboratory
Pathology in Practice 2017 Aug.: 18 (3) 46–7.
https://www.pathologyinpractice.com/features

 

 


• Evaluation of a new lateral flow assay for the detection of VIM-producing bacteria.

P. Bogaerts, S. Evrard, L. Denorme, Q. Gilleman, P. Mertens, T.-D. Huang,1 and Y. Glupczynski.

37e Réunion interdisciplinaire de chimiothérapie anti-infectieuse 18-19 décembre, 2017 Paris..

 Poster

 


 

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