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Products  >  Human-Field  >  OXA-48  >  Science

OXA-48 - Science

 

• Detection of OXA-370 directly from rectal swabs and blood culture vials using an immunochromatographic assay.

Nodari CS, Gales AC, Barth AL, Magagnin CM, Zavascki AP, Carvalhaes CG.

J Microbiol Methods. 2017 May 5;139:92-94. doi: 10.1016/j.mimet.2017.05.003.

Abstract
We evaluated the performance of OXA-48 K-SeT assay for detecting OXA-370 directly from spiked rectal swabs and blood culture vials. The limit of detection of this test was 104UFC/mL for rectal swabs. Detection of the OXA-370-producing isolates was successfully achieved directly from positive blood culture vials independent of growing conditions.

http://www.sciencedirect.com/science/article/pii/S0167701217301136

 

 

• P0292 – Evaluation of five commercial confirmation tests for Carbapenemase-Producing
Enterobacteriaceaein an OXA-48 endemic geographic region.

C. Trouvé, R. De Smedt and E. De Laere

27th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 22 – 25, 2017

 Poster
 

 

• EV0199 – Rapid and accurate detection of OXA-48 like carbapenemases in Enterobacteriaceae using the OXA-48 K-SeT kit.

F. Ismail, P. Meidany, M. Kock, M. Said, N. Mbelle and KA Strydom

27th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 22 – 25, 2017

 Poster

 

 

• Comparison of the Novel Oxa-48 and Kpc K-SeT Assay, and Blue-Carba Test for the Detection of Carbapenemase-Producing Enterobacteriaceae Using PCR as a Reference Method.

Erdem F, Abulaila A, Aktas Z, Oncul O.

Clin Lab. 2017 Mar 1;63(3):515-522. doi: 10.7754/Clin.Lab.2016.160911.

Abstract

BACKGROUND:

Rapid, simple, and accurate laboratory detection of carbapenemases is very important for proper antibiotic therapy and infection control.

METHODS:

In this study, carbapenem-nonsusceptible Enterobacteriaceae (CRE) isolates were used to evaluate the performance of a new lateral flow immunochromatographic (IC) assay, the OXA-48 and KPC K-SeT assay, and modified Blue-Carba test (BCT) for the rapid detection of OXA-48 carbapenemase in comparison with polymerase chain reaction (PCR) amplification. These CREs of various enterobacterial species were isolated from various clinical samples including OXA-48 (47), NDM-1 (6), KPC-1 (1), IMP-1 (1), VIM-2,-4 (2), IMP-2 (1), OXA-51 (1), and OXA-23 (1) producers.

RESULTS:

The OXA-48 K-SeT test detected all OXA-48 carbapenemase producers with 100% sensitivity and specificity. The BCT detected carbapenemase producers with 93% sensitivity and 100% specificity.

CONCLUSIONS:

Both IC assays and BCT tests have good performance and are easy to perform, rapid, simple to interpret, and highly sensitive. We suggest that BCT can be used initially as an accurate, inexpensive, and rapid phenotypic confirmation test to identify Class A, B, and D

https://www.ncbi.nlm.nih.gov/pubmed/28271693


 

• Colistin- and carbapenem-resistant Klebsiella oxytoca harboring blaVIM-2 and an insertion in the mgrB gene isolated from blood culture.

Simon M, Melzl H, Hiergeist A, Richert K, Falgenhauer L, Pfeifer Y, Gerlach RG, Fuchs K, Reischl U, Gessner A, Jantsch J.

Int J Med Microbiol. 2017 Feb;307(2):113-115

Abstract

A carbapenemase-producing colistin-resistant Klebsiella oxytoca isolate was recovered from a blood culture of a female patient without previous report of risk factors to obtain multidrug-resistant Gram-negative bacilli. A combination of biochemical and molecular methods was used to identify the resistance mechanism of this isolate. Carbapenemase production was mediated by Verona integron-encoded metallo-β-lactamase (VIM)-2. Colistin resistance was not due to plasmid- borne mcr-1 gene, but we found an integration of IS5-like sequence in the mgrB gene of K. oxytoca. This gene is known to be an important regulator of the PhoPQ two-component system, and the disruption of this gene is most likely the cause of lipid A modification resulting in colistin resistance of our isolate. To the best of our knowledge this constitutes the first report of a carbapenemase-producing K. oxytoca with colistin resistance, a case that demonstrates the limited treatment options for infections with multidrug-resistant organisms.

https://www.ncbi.nlm.nih.gov/pubmed/28122677


 

• Evaluation of a rapid immunochromatographic test for the detection of OXA-48 carbapenemase.

Rubio E, Zboromyrska Y, Pitart C, Campo I, Alejo-Cancho I, Fasanella A, Vergara A, Marco F, Vila J.

Diagn Microbiol Infect Dis. 2017 Mar;87(3):266-267.

Abstract
We evaluated the OXA-48K-Set, a rapid immunochromatographic test for the detection of Oxacillinase-48 (OXA-48) carbapenemases, among 37 strains expressing OXA-48 and OXA-48-like carbapenemases and 20 additional strains harboring other β-lactamases. The test showed 100% sensitivity and specificity and the results were obtained in 15minutes.

https://www.ncbi.nlm.nih.gov/pubmed/27988171

 


• Comparison of phenotypic tests and an immunochromatographic assay and development of a new algorithm for OXA-48-like detection.

Koroska F, Göttig S, Kaase M, Steinmann J, Gatermann S, Sommer J, Wille T, Plum G1, Hamprecht A.

J Clin Microbiol. 2016 Dec 28. pii: JCM.01929-16. doi: 10.1128/JCM.01929-16. [Epub ahead of print]

Abstract
OXA-48 is the most prevalent carbapenemase in Enterobacteriaceae in Europe and the Middle East, but is frequently missed because many isolates display low minimal inhibitory concentrations (MIC) for carbapenems. Furthermore, in contrast to metallo-β-lactamases or Klebsiella pneumoniae carbapenemases (KPC), no specific inhibitor is available for the phenotypic detection of OXA-48. Molecular detection of blaOXA-48 is the gold standard, but is not available in many laboratories. Few phenotypic assays have been described, but have not been independently evaluated. The aim of this study was the systematic comparison of phenotypic tests and an immunochromatographic assay (ICT) for the detection of OXA-48/OXA-48-like carbapenemases and the development of an algorithm for reliable phenotypic detection of OXA-48.Four phenotypic tests (temocillin disk test, faropenem disk test, OXA-48 disk test and HI OXA-48 disk test) and a new ICT (OXA-48 K-SeT) were compared on a set of 166 Enterobacteriaceae isolates including OXA-48/OXA-48-like (n=84), Ambler class A and B carbapenemases (n=41), and carbapenemase-negative isolates (n=41).The sensitivity and specificity for the different assays was 100%/43.9% for temocillin, 57.1%/98.8% for faropenem, 53.6%/100% for the OXA-48 disk test, 98.8%/97.6% for the HI OXA-48 disk test and 100%/100% for the ICT, respectively.The ICT displayed the highest sensitivity and specificity, was the most rapid assay but is more costly than phenotypic assays. Based on these results, a new algorithm incorporating temocillin, faropenem and ICT was developed, which allows a cost-effective detection of OXA-48 with 100% sensitivity and specificity.

https://www.ncbi.nlm.nih.gov/pubmed/28031433


 

• Evaluation of an immunochromatographic test for the detection of OXA-48 carbapenemase [Article in Spanish].

Mediavilla-Gradolph C, Sáinz-Rodriguez R, Valverde-Troya M, de Toro-Peinado I, Bermudez-Ruíz MP, Palop-Borrás B.Rev Esp Quimioter.

2016 mediavilla 25th november.

Abstract
OBJECTIVE:Detection and differentiation of various types of carbapenemases is crucial to their control and dissemination. OXA-48 is the most common carbapenemase in Spain and in our environment. The aim of this study is the evaluation of a new immunochromatographic test OXA-48 Card letitest (Coris, BioConcept Belgium) to detect this carbapenemase from solid media.
METHODS:During the last year 151 strains of carbapenemase producing bacteria have been isolated, of which 136 were OXA-48 (126 Klebsiella pneumoniae, 1 Klebsiella oxytoca, 5 Escherichia coli, 4 Enterobacter cloacae), and 15 producing other carbapenemases . These 15 strains with other 73 carrying other resistance mechanisms (54 extended-spectrum β-lactamases producers and 19 with other mechanisms) were used as negative controls.
RESULTS:One hundred and thirty six strains carrying OXA-48 were positive with the test OXA-48 Card letitest and the 88 species used as controls were negative, resulting in a sensitivity and specificity of 100%.
CONCLUSIONS:The OXA-48 Card letitest is simple, quick, safe and cheap (approx. 6€/test) and can be used in microbiology laboratories to confirm the production of OXA-48 carbapenemase in clinical isolates.

https://www.ncbi.nlm.nih.gov/pubmed/27897435


 

• Evaluation of the K-SeT R.E.S.I.S.T. immunochromatographic assay for the rapid detecion of KPC and OXA-48-like carbapenemase.

Danièle Meunier, Anna Vickers, Rachel Pike, Robert L.Hill, Neil Woodford, Katie L. Hopkins.

J Antimicrob Chemother. 2016 April 26 doi:10.1093/jac/dkw113

J. Antimicrob. Chemother.-2016-Meunier-jac-dkw113

 


• EP0234 - Evaluation Comparison of a novel OXA-48 K-Set test and blue-carba test in detection of carbapenemase-producing enterobacteriaceae with using PCR as reference method.

A. Abulaila, F. Erdem, Z. Aktas and O. Oncul

26th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 09 – 12, 2016

 Poster234


 

• P0946 - The Coris BioConcept OXA48 K-SeT Immuno-Chromatographic Assay Detects OXA48-type Carbapenemases with High Sensitivity and Specificity.

B.M. Willey, X. Trimi, R. Ioboni, D.A. Boyd, G. Ricci, D.N. Grohn, D. Terenzi, A. Mazzulli, L. Mataseje, M. Mulvey, P. Lo, T. Mazzulli, S.M. Poutanen

26th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 09 – 12, 2016

 Poster946


 

• EV0432 - Evaluation of the performance of OXA-48 K-SeT immunochromatographic test for rapid identification of OXA-48 carbapenemase-producing Enterobacteriaceae.

O. Karatuna, M. Kaya, I. Akyar

26th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 09 – 12, 2016

 Poster432


 

• EV0445 - OXA-370 is rapidly detected from different culture media using OXA-48 K-SeT® immunochromatography.

C.S. Nodari, A. Barth, C. Magagnin, A. Zavascki, A. Gales, C. Carvalhaes

26th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 09 – 12, 2016

 Poster445


 

• P0840 - Evaluation of a rapid diagnostic test for the detection of OXA-48 carbapenemase

E. Rubio, Y. Zboromyrska, C. Pitart, I. Alejo-Cancho, I. Campo, A. Fasanella1, F. M. Reverte, J. Vila.

26th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 09 – 12, 2016

 Poster840


 

• EV0447 - A rapid test for detection of OXA-48 carbapenemase.

I. De Toro Peinado, MªC.M. Gradolph, R. S. Rodriguez, M. V. Troya, MªP. B. Ruiz, B. Palop

26th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 09 – 12, 2016

 Poster447

 


• EV0186 - Preliminay study of the OXA-48 card letitest method for the direct detection of OXA-48 carbapenemase in blood and plates culture.

A. Sarria, R. Gomez-Gil, G. Ruiz, MP. Romero, J. García- Rodríguez

26th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 09 – 12, 2016

 Poster186

 


• EV0462 – Specificity of the OXA-48 immunochromatographic K-SeT for the detection of OXA-48 like in Shewanella spp.

P. Bogaerts, S. Evrard, G. Cuzon, TD. Huang, T. Naas and Y. Glupczynski

26th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 09 – 12, 2016

 Poster462

 


• P1022 – Impact of the isolation medium for the detection of OXA-48 and KPC-producing Gram negative bacteria by immunochromatographic assays.

P. Bogaerts, S. Evrard, M. Dozen, TD. Huang and Y. Glupczynski

26th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 09 – 12, 2016

 Poster1022


 

• P0952 - Prospective evaluation of the OXA-48 K-SeT for the detection of OXA-48-type carbapenemase producing Enterobacteriaceae.

L. Dortet, A. Jousset, V. Sainte-Rose, G. Cuzon, and T. Naas

26th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 09 – 12, 2016

 Poster186


 

• Evaluation of OXA-48 K-SeT: an immunochromatographic assay for rapid detection of OXA-48-producing enteriobacteriaceae. 

Fernandez J, Fleites A, Rodcio MR, Vazquez F.

Diagn Micribiol Infect Dis. 2016 Jan 26. pii: S0732-8893(16)00034-1. doi: 10.1016/j.diagmicrobio.2016.01.015. [Epub ahead of print].

http://www.ncbi.nlm.nih.gov/pubmed/26971639


 

• Prospective evaluation of the OXA-48 K-SeT assay, an immunochromatographic test for the rapid detection of OXA-48-type carbapenemases. 

Dortet L, Jousset A, Sainte-Rose V, Cuzon G, Naas T.

J Antimicrob Chemother. 2016 Mar 10. pii: [Epub ahead of print].

http://www.ncbi.nlm.nih.gov/pubmed/26968882

 

 

• Evaluation du kit OXA-48 K-Set (CORIS BioConcept) pour la détection des souches d’entérobactéries productrices de carbapénémases de type OXA-48 isolées en Algérie. 

N.Aggoune, H.Tali-Maamar, B.Guettou , A.Zerouki , M.Naim, K.Rahal.

35e Réunion interdisciplinaire de chimiothérapie anti-infectieuse 13 - 15 décembre, 2015 Paris.

 Paris 2015 - Evaluation OXA-48

 

• Evaluation of two new commercial immunochromatographic assays for the rapid detection of OXA-48 and KPC carbapenemases from cultured bacteria. 

Glupczynski Y, Evrard S, Ote I, Mertens P, Huang TD, Leclipteux T, Bogaerts P.

J Antimicrob Chemother. 2016 Jan 28. pii: dkv472. [Epub ahead of print]

http://www.ncbi.nlm.nih.gov/pubmed/26825120

Abstract :
BACKGROUND:
Rapid detection and confirmation of carbapenemases remains very challenging for diagnostic laboratories.
OBJECTIVES:
The objective of this study was to assess the performance of two new immunochromatographic (IC) commercial assays for the rapid detection of OXA-48-producing and KPC-producing Enterobacteriaceae in pure bacterial isolates.
METHODS:
A panel of 92 bacterial isolates predominantly including carbapenem-non-susceptible Enterobacteriaceae with previously defined carbapenem resistance mechanisms was tested. Then, 342 consecutive carbapenem-non-susceptible Enterobacteriaceae isolates referred to the reference laboratory were investigated prospectively in parallel with other phenotypic tests and with multiplex PCR and sequencing as the gold standard.
RESULTS:
In the collection panel, each of the two IC assays correctly detected all 30 OXA-48-like-producing isolates and 25 KPC-producing isolates, whatever the species, their association with other β-lactamases and the level of resistance to carbapenems. All other carbapenemase producers and all non-carbapenemase-producing isolates yielded negative results with both tests. In the prospective evaluation, all OXA-48-like-producing Enterobacteriaceae isolates (n = 130) and KPC-producing Enterobacteriaceae isolates (n = 33) were correctly detected by the individual IC assays, while 179 non-OXA-48-like-producing and non-KPC-producing strains (137 non-carbapenemase producers and 42 isolates belonging to other carbapenemase family types) yielded negative results. Thus, each assay yielded 100% sensitivity and 100% specificity for the detection of OXA-48-like or KPC enzymes, respectively, at 15 min.
CONCLUSIONS:
The two IC assays allow rapid and reliable direct confirmation of OXA-48 and KPC carbapenemases from culture colonies and appear to be very useful additions to the existing tests, obviating the need for more costly characterization by molecular amplification methods.

 

• Prospective evaluation of the OXA-48 K-SeT for the detection of OXA-48-type carbapenemase producing Enterobacteriaceae. 

Laurent Dortet, Agnès Jousset, Vincent Sainte-Rose, Gaëlle Cuzon and Thierry Naas.

55th ICAAC 2015 September 17-21, 2015  San Diego, CA.

 ICAAC - 2015

 

• Evaluation of an Immunochromatographic Lateral Flow Assay (OXA-48 K-SeT) for the Rapid Detection of OXA-48-like Carbapenemases in Enterobacteriaceae. 

Wareham DW, Shah R, Betts JW, Phee LM, Abdul Momin MH.

J Clin Microbiol. 2015 Nov 25. pii: JCM.02900-15.

http://www.ncbi.nlm.nih.gov/pubmed/26607983

Abstract : 
We evaluated an immunochromatographic lateral flow assay for the detection of OXA-48-like carbapenemases (OXA-48 K-SeT) in Enterobacteriaceae (n=82). 100% sensitivity and specificity was observed using bacteria recovered from both solid media and spiked blood culture bottles, with the result obtained in less than 10 minutes.

 

 

• Development of a novel immunochromatographic combo test for rapid identification of OXA-­48 and KPC carbapenemases in Enterobacteriaceae. 

Céline Borlon, Laurence Denorme, Stéphanie Evrard, Isabelle Ote, Pierre Bogaerts, Youri Glupczynski, Pascal Mertens.

115th American Society for Microbiology May 30 - June 2, 2015 New Orleans.

 ASM 2015

 

 

• Development of a novel immunochromatographic confirmatory test for the detection of OXA-­48 carbapenemase in Enterobacteriaceae.

Isabelle Ote, Pierre Bogaerts, Laurence Denorme, Céline Borlon, Caroline Thunissen, Youri Glupczynski, Thierry Leclipteux, Pascal Mertens.

25th European Congress of Clinical Microbiology and Infectious Diseases April-25 - 28, 2015

Development of a novel...

 ECCMID presentation - Isabelle OTE


 

• Validation of a new lateral flow assay for the detection of OXA-48-like-producing Enterobacteriaceae

Pierre Bogaerts

25th European Congress of Clinical Microbiology and Infectious Diseases April-25 - 28, 2015

ECCMID 2015

http://www.eccmidlive.org/

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